Universität zu Köln

Hos, Nina J., Rieg, Siegbert ORCID: 0000-0001-7493-7080, Kern, Winfried V., Jonas, Daniel, Fowler, Vance G., Higgins, Paul G. ORCID: 0000-0001-8677-9454, Seifert, Harald and Kaasch, Achim J. (2015). Amino acid alterations in fibronectin binding protein A (FnBPA) and bacterial genotype are associated with cardiac device related infection in Staphylococcus aureus bacteraemia. J. Infect., 70 (2). S. 153 - 160. LONDON: W B SAUNDERS CO LTD. ISSN 1532-2742

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Abstract

Objectives: Staphylococcus aureus initiates cardiac device-related infection (CDI) by binding of fibronectin binding protein A (FnBPA) to the device's surface. In FnBPA, specific binding enhancing amino acid alterations are associated with CDI. However, no study has investigated whether these mutations also occur in geographically different regions and whether they arise during infection or are inherent properties of the infecting isolate. Methods: We analysed bacterial isolates from 34 patients with S. aureus bacteraemia and implanted cardiac devices for association with CDI, FnBPA sequence, classification into a clonal complex (CC), and binding to fibronectin (Fn). Results: We confirmed that amino acid alterations at positions 652, 782, and 786 in FnBPA were associated with CDI (p = 0.005). Furthermore, CC15 and CC45 isolates were associated with CDI (p = 0.004). All isolates within a CC exhibited a characteristic mutation pattern, with major changes occurring in CC45 including a duplication of D1 and an altered immunogenic epitope in the D3 repeat. Isolates harbouring the binding enhancing mutations showed a slightly increased Fn binding capability, whereas Fn binding was decreased in CC45 isolates, according to a microtiter plate assay. Conclusions: FnBPA sequence variations are lineage specific and display inherent properties of the infecting isolate. Sequence analysis of FnBPA, as well as the bacterial genotype, may be used to predict the risk for device-related infection. (C) 2014 The British Infection Association. Published by Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hos, Nina J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Rieg, Siegbert UNSPECIFIED orcid.org/0000-0001-7493-7080 UNSPECIFIED Kern, Winfried V. UNSPECIFIED UNSPECIFIED UNSPECIFIED Jonas, Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED Fowler, Vance G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Higgins, Paul G. UNSPECIFIED orcid.org/0000-0001-8677-9454 UNSPECIFIED Seifert, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaasch, Achim J. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-413996
DOI:	10.1016/j.jinf.2014.09.005
Journal or Publication Title:	J. Infect.
Volume:	70
Number:	2
Page Range:	S. 153 - 160
Date:	2015
Publisher:	W B SAUNDERS CO LTD
Place of Publication:	LONDON
ISSN:	1532-2742
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TANDEM BETA-ZIPPER; EXPERIMENTAL ENDOCARDITIS; NUCLEOTIDE-SEQUENCE; GENES; POLYMORPHISMS; ASSIGNMENT; MANAGEMENT; FIBRINOGEN; ADHERENCE; DIAGNOSIS Multiple languages Infectious Diseases Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41399