Universität zu Köln

Figueroa, E., Merino, O., Risopatron, J., Isachenko, V., Sanchez, R., Effer, B., Isachenko, E., Farias, J. G. and Valdebenito, I. (2015). Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification. Theriogenology, 83 (2). S. 238 - 246. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1879-3231

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Abstract

This study was designed to test a vitrification method in Atlantic salmon spermatozoa and determine the capacity of seminal plasma (SP) to protect these cells from cryoinjuries. The vitrification medium consisted of a standard buffer for fish spermatozoa (Cortland medium) + 10% DMSO + 2% BSA + 0.13-M sucrose + SP at concentrations of 30% (G30), 40% (G40), or 50% (G50). Fresh sperm was used as a control. To freeze the samples, 30-mu L suspensions of spermatozoa from each group were dropped directly into liquid nitrogen. The resulting spheres were placed in cryotubes for storage in liquid nitrogen. The cryotubes with the vitrified spermatozoa were thawed by placing them in a water bath at 37 degrees C for 45 seconds. After thawing, the following sperm quality parameters were determined by flow cytometry: DNA fragmentation (terminal deoxynucleotidyl transferase dUTP nick end labeling), plasma membrane integrity (SYBR-14/PI, staining technique), and mitochondrial membrane potential (JC-1 staining). An optical microscope was used to assess subjectively sperm motility, whereas fertility was determined by the presence of neurulation using five replicates per treatment in a sample of 30 eggs. Spermatozoa quality variables were preserved best when the highest concentration of SP (50%) was used (DNA fragmentation, 9.2%; plasma membrane integrity, 98.6%; mitochondrial membrane integrity, 47.2%; motility, 44.1%; and fertility, 46.2%). (C) 2015 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Figueroa, E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Merino, O. UNSPECIFIED UNSPECIFIED UNSPECIFIED Risopatron, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, V. UNSPECIFIED UNSPECIFIED UNSPECIFIED Sanchez, R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Effer, B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Farias, J. G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Valdebenito, I. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-414677
DOI:	10.1016/j.theriogenology.2014.09.015
Journal or Publication Title:	Theriogenology
Volume:	83
Number:	2
Page Range:	S. 238 - 246
Date:	2015
Publisher:	ELSEVIER SCIENCE INC
Place of Publication:	NEW YORK
ISSN:	1879-3231
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CRYOPROTECTANT-FREE VITRIFICATION; RAINBOW-TROUT; SUPEROXIDE-DISMUTASE; HUMAN-SPERMATOZOA; OXIDATIVE STRESS; FERTILIZING-CAPACITY; CRYOPRESERVATION; FISH; MOTILITY; SEMEN Multiple languages Reproductive Biology; Veterinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41467