Universität zu Köln

Hinkelbein, Jochen, Boehm, Lennert, Spelten, Oliver, Sander, David, Soltesz, Stefan and Braunecker, Stefan ORCID: 0000-0003-3095-764X (2015). Hyperoxia-Induced Protein Alterations in Renal Rat Tissue: A Quantitative Proteomic Approach to Identify Hyperoxia-Induced Effects in Cellular Signaling Pathways. Dis. Markers, 2015. S. 1 - 13. LONDON: HINDAWI LTD. ISSN 1875-8630

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Abstract

Introduction. In renal tissue as well as in other organs, supranormal oxygen pressure may lead to deleterious consequences on a cellular level. Additionally, hyperoxia-induced effect in cells and related free radicals may potentially contribute to renal failure. The aim of this study was to analyze time-dependent alterations of rat kidney protein expression after short-term normobaric hyperoxia using proteomics and bioinformatic approaches. Material and Methods. N = 36 Wistar rats were randomized into six different groups: three groups with normobaric hyperoxia (exposure to 100% oxygen for 3 h) and three groups with normobaric normoxia (NN; room air). After hyperoxia exposure, kidneys were removed immediately, after 3 days and after 7 days. Kidney lysates were analyzed by two-dimensional gel electrophoresis followed by peptide mass fingerprinting using tandem mass spectrometry. Statistical analysis was performed with DeCyder 2D software (p < 0.01). Biological functions of differential regulated proteins were studied using functional network analysis (Ingenuity Pathways Analysis and PathwayStudio). Results. Expression of 14 proteins was significantly altered (p < 0.01): eight proteins (MEP1A_RAT, RSSA_RAT, F16P1_RAT, STML2_RAT, BPNT1_RAT, LGMN_RAT, ATPA_RAT, and VDAC1_RAT) were downregulated and six proteins (MTUS1_RAT, F16P1_RAT, ACTG_RAT, ACTB_RAT, 2ABA_RAT, and RAB1A_RAT) were upregulated. Bioinformatic analyses revealed an association of regulated proteins with inflammation. Conclusions. Significant alterations in renal protein expression could be demonstrated for up to 7 days even after short-term hyperoxia. The identified proteins indicate an association with inflammation signaling cascades. MEP1A and VDAC1 could be promising candidates to identify hyperoxic injury in kidney cells.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hinkelbein, Jochen UNSPECIFIED UNSPECIFIED UNSPECIFIED Boehm, Lennert UNSPECIFIED UNSPECIFIED UNSPECIFIED Spelten, Oliver UNSPECIFIED UNSPECIFIED UNSPECIFIED Sander, David UNSPECIFIED UNSPECIFIED UNSPECIFIED Soltesz, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Braunecker, Stefan UNSPECIFIED orcid.org/0000-0003-3095-764X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-416411
DOI:	10.1155/2015/964263
Journal or Publication Title:	Dis. Markers
Volume:	2015
Page Range:	S. 1 - 13
Date:	2015
Publisher:	HINDAWI LTD
Place of Publication:	LONDON
ISSN:	1875-8630
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SUPEROXIDE-DISMUTASE; LUNG INJURY; MEPRIN-A; OXYGEN; EXPRESSION; DEATH; APOPTOSIS; KIDNEY; METALLOPROTEINASE; METABOLOME Multiple languages Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental; Pathology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41641