Universität zu Köln

Alili, Lirija, Chapiro, Swetlana, Marten, Gernot U., Schmidt, Annette M., Zanger, Klaus and Brenneisen, Peter ORCID: 0000-0003-3405-0287 (2015). Effect of Fe3O4 Nanoparticles on Skin Tumor Cells and Dermal Fibroblasts. Biomed Res. Int., 2015. LONDON: HINDAWI LTD. ISSN 2314-6141

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Abstract

Iron oxide (Fe3O4) nanoparticles have been used in many biomedical approaches. The toxicity of Fe3O4 nanoparticles on mammalian cells was published recently. Though, little is known about the viability of human cells after treatment with Fe3O4 nanoparticles. Herein, we examined the toxicity, production of reactive oxygen species, and invasive capacity after treatment of human dermal fibroblasts (HDF) and cells of the squamous tumor cell line (SCL-1) with Fe3O4 nanoparticles. These nanoparticles had an average size of 65 nm. Fe3O4 nanoparticles induced oxidative stress via generation of reactive oxygen species (ROS) and subsequent initiation of lipid peroxidation. Furthermore, the question was addressed of whether Fe3O4 nanoparticles affect myofibroblast formation, known to be involved in tumor invasion. Herein, Fe3O4 nanoparticles prevent the expression alpha-smooth muscle actin and therefore decrease the number of myofibroblastic cells. Moreover, our data show in vitro that concentrations of Fe3O4 nanoparticles, which are nontoxic for normal cells, partially reveal a ROS-triggered cytotoxic but also a pro-invasive effect on the fraction of squamous cancer cells surviving the treatment with Fe3O4 nanoparticles. The data herein show that the Fe3O4 nanoparticles appear not to be adequate for use in therapeutic approaches against cancer cells, in contrast to recently published data with cerium oxide nanoparticles.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Alili, Lirija UNSPECIFIED UNSPECIFIED UNSPECIFIED Chapiro, Swetlana UNSPECIFIED UNSPECIFIED UNSPECIFIED Marten, Gernot U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Annette M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Zanger, Klaus UNSPECIFIED UNSPECIFIED UNSPECIFIED Brenneisen, Peter UNSPECIFIED orcid.org/0000-0003-3405-0287 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-416534
DOI:	10.1155/2015/530957
Journal or Publication Title:	Biomed Res. Int.
Volume:	2015
Date:	2015
Publisher:	HINDAWI LTD
Place of Publication:	LONDON
ISSN:	2314-6141
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IRON-OXIDE NANOPARTICLES; OXIDATIVE STRESS; IN-VITRO; MAGNETIC NANOPARTICLES; GRANULATION-TISSUE; MYOFIBROBLASTS; PROTEINS; INVASION; DEXTRAN; STROMA Multiple languages Biotechnology & Applied Microbiology; Medicine, Research & Experimental Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41653