Universität zu Köln

Kosmas, Kosmas, Eskandarnaz, Ali, Khorsandi, Arya B., Kumar, Atul, Ranjan, Rajeev, Eming, Sabine A., Noegel, Angelika A. and Peche, Vivek S. (2015). CAP2 is a regulator of the actin cytoskeleton and its absence changes infiltration of inflammatory cells and contraction of wounds. Eur. J. Cell Biol., 94 (1). S. 32 - 46. JENA: ELSEVIER GMBH, URBAN & FISCHER VERLAG. ISSN 1618-1298

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Abstract

Cyclase associated protein (CAP) is a highly conserved protein with roles in actin dynamics and many cellular processes. Two isoforms exist in higher eukaryotes, CAP1 and CAP2. CAP1 is ubiquitously expressed whereas CAP2 shows restricted tissue distribution. In mice, ablation of CAP2 leads to development of cardiomyopathy. CAP2 is expressed in skin. In human skin its expression is increased in wounds. To elucidate the role of CAP2 in skin upon injury, we studied the wound healing in CAP2 deficient mice and found altered wound healing response presumably resulting from reduced levels of alpha-SMA, decreased macrophage infiltration and slower neovascularization. In vitro cultured Cap2 deficient keratinocytes showed reduced velocity and a delay in scratch closure. The analysis of primary mutant fibroblasts also showed reduced velocity and less contractibility. They had extended protrusions and more focal adhesions. In addition the F-actin content was increased keeping the total actin content unaltered. Mutant fibroblasts furthermore exhibited an altered response during recovery from drug-induced disruption of the actin cytoskeleton. Interestingly, CAP1 was upregulated in knockout unwounded skin and in wounds which might partially compensate for the loss of CAP2. Taken together, our studies reveal a role for CAP2 in wound healing which may be based on its function as a regulator of the actin cytoskeleton. (C) 2014 Elsevier GmbH. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kosmas, Kosmas UNSPECIFIED UNSPECIFIED UNSPECIFIED Eskandarnaz, Ali UNSPECIFIED UNSPECIFIED UNSPECIFIED Khorsandi, Arya B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kumar, Atul UNSPECIFIED UNSPECIFIED UNSPECIFIED Ranjan, Rajeev UNSPECIFIED UNSPECIFIED UNSPECIFIED Eming, Sabine A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Noegel, Angelika A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Peche, Vivek S. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-417096
DOI:	10.1016/j.ejcb.2014.10.004
Journal or Publication Title:	Eur. J. Cell Biol.
Volume:	94
Number:	1
Page Range:	S. 32 - 46
Date:	2015
Publisher:	ELSEVIER GMBH, URBAN & FISCHER VERLAG
Place of Publication:	JENA
ISSN:	1618-1298
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CYCLASE-ASSOCIATED PROTEIN; MYOFIBROBLAST DIFFERENTIATION; ADENYLATE-CYCLASE; REPAIR; MECHANISMS; YEAST; FIBROBLASTS; MACROPHAGES; INDUCTION; ABLATION Multiple languages Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41709