Universität zu Köln

Manthou, M., Nohroudi, K., Moscarino, S., Rehberg, F., Stein, G., Jansen, R., Abdulla, D., Jaminet, P., Semler, O., Schoenau, E. and Angelov, D. N. (2015). Functional recovery after experimental spinal cord compression and whole body vibration therapy requires a balanced revascularization of the injured site. Restor. Neurol. Neurosci., 33 (2). S. 233 - 250. AMSTERDAM: IOS PRESS. ISSN 1878-3627

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Abstract

Purpose: Based on several positive effects of whole-body-vibration (WBV) therapy on recovery after SCI, we looked for correlations between functional (analysis of locomotion), electrophysiological (H-reflex) and morphological (density of functioning capillaries) measurements after SCI and WBV-treatment. Methods: Severe compression SCI at low-thoracic level (T8) in adult female Wistar rats was followed by WBV twice a day (2xWBV) over a 12-week post-injury period. Intact rats and rats with SCI but no WBV-therapy (No-WBV) served as controls. Recovery of locomotion was determined by BBB-locomotor rating, foot stepping angle (FSA), rump-height index (RHI), correct ladder steps (CLS) and H-reflex at 1, 3, 6, 9, and 12 weeks after SCI. Animals were sacrificed by an overdose of Isoflurane (Abbott). One hour later their spinal cords were fixed in 4% PFA for 24 h. Samples from the thoracic cord containing the lesion site and from the lumbar intumescence were cut into 10 mu m thick longitudinal frozen sections. Results: All functioning capillaries were unequivocally identified because the endogenous peroxidase of the erythrocytes was clearly visualized with 0.05% diaminobenzidine (DAB). A determination of their absolute (in mu m(2)) and proportional areas (percent of photographed tissue) revealed a significantly denser capillary network in the WBV-treated rats: 1,66 +/- 0,41% in the vibrated rats versus 0,79 +/- 0,19% in the No-WBV animals. The portion of the capillary network in intact rats was 1,51 +/- 0,69%. Surprisingly, even though the vascularization in the treated animals was significantly increased, this had no beneficial influence on the recovery of functions after SCI. Conclusion: The results of this study provide for the first time evidence that intensive WBV-therapy leads to a significantly denser capillary network in the lesioned spinal cord. However, since this higher capillary density is not associated with improved functional recovery (possibly because it exceeded the balance necessary for functional improvements), optional treatments with lower intensity or less time of WBV-therapy should be tested.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Manthou, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Nohroudi, K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Moscarino, S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Rehberg, F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Stein, G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Jansen, R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Abdulla, D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Jaminet, P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Semler, O. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schoenau, E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Angelov, D. N. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-417272
DOI:	10.3233/RNN-140434
Journal or Publication Title:	Restor. Neurol. Neurosci.
Volume:	33
Number:	2
Page Range:	S. 233 - 250
Date:	2015
Publisher:	IOS PRESS
Place of Publication:	AMSTERDAM
ISSN:	1878-3627
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FIBROBLAST-GROWTH-FACTOR; BLOOD-FLOW; H-REFLEX; EXERCISE; RESPONSES; MUSCLE; PERFORMANCE; ANGIOGENESIS; INDIVIDUALS; WORLDWIDE Multiple languages Neurosciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41727