Universität zu Köln

Deckstein, Jaqueline, van Appeldorn, Jennifer, Tsangarides, Marios, Yiannakou, Kyriacos, Mueller, Rolf, Stumpf, Maria, Sukumaran, Salil K., Eichinger, Ludwig ORCID: 0000-0003-1594-6117, Noegel, Angelika A. and Riyahi, Tanja Y. (2015). The Dictyostelium discoideum GPHR Ortholog Is an Endoplasmic Reticulum and Golgi Protein with Roles during Development. Eukaryot. Cell, 14 (1). S. 41 - 55. WASHINGTON: AMER SOC MICROBIOLOGY. ISSN 1535-9786

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Abstract

Dictyostelium discoideum GPHR (Golgi pH regulator)/Gpr89 is a developmentally regulated transmembrane protein present on the endoplasmic reticulum (ER) and the Golgi apparatus. Transcript levels are low during growth and vary during development, reaching high levels during the aggregation and late developmental stages. The Arabidopsis ortholog was described as a G protein-coupled receptor (GPCR) for abscisic acid present at the plasma membrane, whereas the mammalian ortholog is a Golgi apparatus-associated anion channel functioning as a Golgi apparatus pH regulator. To probe its role in D. discoideum, we generated a strain lacking GPHR. The mutant had different growth characteristics than the AX2 parent strain, exhibited changes during late development, and formed abnormally shaped small slugs and fruiting bodies. An analysis of development-specific markers revealed that their expression was disturbed. The distributions of the endoplasmic reticulum and the Golgi apparatus were unaltered at the immunofluorescence level. Likewise, their functions did not appear to be impaired, since membrane proteins were properly processed and glycosylated. Also, changes in the external pH were sensed by the ER, as indicated by a pH-sensitive ER probe, as in the wild type.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Deckstein, Jaqueline UNSPECIFIED UNSPECIFIED UNSPECIFIED van Appeldorn, Jennifer UNSPECIFIED UNSPECIFIED UNSPECIFIED Tsangarides, Marios UNSPECIFIED UNSPECIFIED UNSPECIFIED Yiannakou, Kyriacos UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Rolf UNSPECIFIED UNSPECIFIED UNSPECIFIED Stumpf, Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Sukumaran, Salil K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Eichinger, Ludwig UNSPECIFIED orcid.org/0000-0003-1594-6117 UNSPECIFIED Noegel, Angelika A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Riyahi, Tanja Y. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-417401
DOI:	10.1128/EC.00208-14
Journal or Publication Title:	Eukaryot. Cell
Volume:	14
Number:	1
Page Range:	S. 41 - 55
Date:	2015
Publisher:	AMER SOC MICROBIOLOGY
Place of Publication:	WASHINGTON
ISSN:	1535-9786
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ACTIN-BINDING PROTEIN; SITE-A GLYCOPROTEIN; FUNCTIONAL-CHARACTERIZATION; CELL POLARITY; PH; MEMBRANE; POLYMERIZATION; IDENTIFICATION; HISACTOPHILIN; DISRUPTION Multiple languages Microbiology; Mycology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41740