Universität zu Köln

Lienemann, P. S., Devaud, Y. R., Reuten, R., Simona, B. R., Karlsson, M., Weber, W., Koch, M., Lutolf, M. P., Milleret, V. and Ehrbar, M. (2015). Locally controlling mesenchymal stem cell morphogenesis by 3D PDGF-BB gradients towards the establishment of an in vitro perivascular niche. Integr. Biol., 7 (1). S. 101 - 112. OXFORD: OXFORD UNIV PRESS. ISSN 1757-9708

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Abstract

The perivascular niche is a complex microenvironment containing mesenchymal stem cells (MSCs), among other perivascular cells, as well as temporally organized biochemical and biophysical gradients. Due to a lack of conclusive phenotypic markers, MSCs' identity, heterogeneity and function within their native niche remain poorly understood. The in vitro reconstruction of an artificial three-dimensional (3D) perivascular niche would offer a powerful alternative to study MSC behavior under more defined conditions. To this end, we here present a poly(ethylene glycol)-based in vitro model that begins to mimic the spatiotemporally controlled presentation of biological cues within the in vivo perivascular niche, namely a stably localized platelet-derived growth factor B (PDGF-BB) gradient. We show that 3D-encapsulated MSCs respond to soluble PDGF-BB by proliferation, spreading, and migration in a dose-dependent manner. In contrast, the exposure of MSCs to 3D matrix-tethered PDGF-BB gradients resulted in locally restricted morphogenetic responses, much as would be expected in a native perivascular niche. Thus, the herein presented artificial perivascular niche model provides an important first step towards modeling the role of MSCs during tissue homeostasis and regeneration.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lienemann, P. S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Devaud, Y. R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Reuten, R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Simona, B. R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Karlsson, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Weber, W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Koch, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lutolf, M. P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Milleret, V. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ehrbar, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-417740
DOI:	10.1039/c4ib00152d
Journal or Publication Title:	Integr. Biol.
Volume:	7
Number:	1
Page Range:	S. 101 - 112
Date:	2015
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1757-9708
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ENDOTHELIAL GROWTH-FACTOR; IMMOBILIZED GRADIENTS; VASCULAR NICHE; VEGF; PERICYTES; HYDROGELS; ANGIOGENESIS; MECHANISM; MIGRATION; MATRIX Multiple languages Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41774