Universität zu Köln

Anadol, Evrim, Schierwagen, Robert ORCID: 0000-0002-2195-3666, Elfimova, Natalia, Tack, Katharina, Schwarze-Zander, Carolynne, Eischeid, Hanna, Noetel, Andrea, Boesecke, Christoph, Jansen, Christian, Dold, Leona, Wasmuth, Jan-Christian, Strassburg, Christian P., Spengler, Ulrich, Rockstroh, Juergen Kurt, Odenthal, Margarete and Trebicka, Jonel ORCID: 0000-0002-7028-3881 (2015). Circulating MicroRNAs as a Marker for Liver Injury in Human Immunodeficiency Virus Patients. Hepatology, 61 (1). S. 46 - 56. HOBOKEN: WILEY. ISSN 1527-3350

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Abstract

Human immunodeficiency virus (HIV) and hepatitis virus coinfection amplify and accelerate hepatic injury. MicroRNAs (miRNAs) are small regulatory RNAs suggested as biomarkers for liver injury. We analyzed the circulating levels of miRNAs in HIV patients with regard to the extent and etiology of liver injury. Total RNA was extracted from 335 serum samples of HIV patients and 22 healthy control participants using Qiazol. Comprehensive polymerase chain reaction (PCR) array analyses (768 miRNA) were performed in serum samples of eight HIV, eight HIV/HCV (hepatitis C virus), six HCV patients, and three healthy controls. Reverse transcription (RT)-PCR measured levels of miRNA-122, miRNA-22, and miRNA-34a in serum samples of 335 patients and 19 healthy control participants. Liver injury and fibrosis in these patients were defined using aspartate aminotransferase (AST) levels, fibrosis-4 (FIB-4) index and AST-to-platelet ratio index (APRI) score. The miRNA pattern of HIV/HCV samples showed altered expression of 57 and 33 miRNA compared to HCV and HIV infection, respectively. miRNA-122, miRNA-22, and miRNA-34a were highly up-regulated in HIV/HCV patients. Analyzing the entire cohort, these miRNAs were correlated with liver function tests and were independent predictors of liver injury (AST >2 x ULN). miRNA-122 and miRNA-22 were associated with relevant fibrosis (FIB-4 >1.45; APRI >1). Circulating levels of miRNA-122 were independent predictors for relevant fibrosis in HIV patients. Interestingly, miRNA-122 and miRNA-34a levels were higher in HIV/HCV patients, miRNA-22 levels were highest in HIV/HBV patients, and circulating levels of miRNA-34a correlated positively with illicit drug use and ethanol consumption. Conclusion: Circulating miRNA-122, miRNA-22, and miRNA-34a correlates with the etiology of liver injury in HIV patients. These biomarkers not only mirror different mechanisms of hepatic injury, but also are independent predictors of liver injury in HIV patients. (Hepatology 2015;61:46-55)

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Anadol, Evrim UNSPECIFIED UNSPECIFIED UNSPECIFIED Schierwagen, Robert UNSPECIFIED orcid.org/0000-0002-2195-3666 UNSPECIFIED Elfimova, Natalia UNSPECIFIED UNSPECIFIED UNSPECIFIED Tack, Katharina UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwarze-Zander, Carolynne UNSPECIFIED UNSPECIFIED UNSPECIFIED Eischeid, Hanna UNSPECIFIED UNSPECIFIED UNSPECIFIED Noetel, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Boesecke, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Jansen, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Dold, Leona UNSPECIFIED UNSPECIFIED UNSPECIFIED Wasmuth, Jan-Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Strassburg, Christian P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Spengler, Ulrich UNSPECIFIED UNSPECIFIED UNSPECIFIED Rockstroh, Juergen Kurt UNSPECIFIED UNSPECIFIED UNSPECIFIED Odenthal, Margarete UNSPECIFIED UNSPECIFIED UNSPECIFIED Trebicka, Jonel UNSPECIFIED orcid.org/0000-0002-7028-3881 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-418964
DOI:	10.1002/hep.27369
Journal or Publication Title:	Hepatology
Volume:	61
Number:	1
Page Range:	S. 46 - 56
Date:	2015
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1527-3350
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HEPATITIS-C VIRUS; SIMPLE NONINVASIVE INDEX; HIV-INFECTED PATIENTS; FIBROSIS PROGRESSION; RISK-FACTORS; MONOINFECTED PATIENTS; HIV/HCV COINFECTION; EXPRESSION; DISEASE; THERAPY Multiple languages Gastroenterology & Hepatology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41896