Universität zu Köln

Kamp, Marcel A., Dibue, Maxine, Sommer, Clemens, Steiger, Hans-Jakob, Schneider, Toni and Haenggi, Daniel (2014). Evaluation of a Murine Single-Blood-Injection SAH Model. PLoS One, 9 (12). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

The molecular pathways underlying the pathogenesis after subarachnoid haemorrhage (SAH) are poorly understood and continue to be a matter of debate. A valid murine SAH injection model is not yet available but would be the prerequisite for further transgenic studies assessing the mechanisms following SAH. Using the murine single injection model, we examined the effects of SAH on regional cerebral blood flow (rCBF) in the somatosensory (S1) and cerebellar cortex, neuro-behavioural and morphological integrity and changes in quantitative electrocorticographic and electrocardiographic parameters. Micro CT imaging verified successful blood delivery into the cisterna magna. An acute impairment of rCBF was observed immediately after injection in the SAH and after 6, 12 and 24 hours in the S1 and 6 and 12 hours after SAH in the cerebellum. Injection of blood into the foramen magnum reduced telemetric recorded total ECoG power by an average of 65%. Spectral analysis of ECoGs revealed significantly increased absolute delta power, i.e., slowing, cortical depolarisations and changes in ripples and fast ripple oscillations 12 hours and 24 hours after SAH. Therefore, murine single-blood-injection SAH model is suitable for pathophysiological and further molecular analysis following SAH.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kamp, Marcel A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Dibue, Maxine UNSPECIFIED UNSPECIFIED UNSPECIFIED Sommer, Clemens UNSPECIFIED UNSPECIFIED UNSPECIFIED Steiger, Hans-Jakob UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, Toni UNSPECIFIED UNSPECIFIED UNSPECIFIED Haenggi, Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-419965
DOI:	10.1371/journal.pone.0114946
Journal or Publication Title:	PLoS One
Volume:	9
Number:	12
Date:	2014
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXPERIMENTAL SUBARACHNOID HEMORRHAGE; DELAYED CEREBRAL-ISCHEMIA; SPREADING DEPOLARIZATION; PERFUSION-PRESSURE; CONTINUOUS EEG; MOUSE MODEL; IN-VIVO; FLOW; VASOSPASM; BRAIN Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/41996