Universität zu Köln

Lu, Yameng, Wohlrab, Sylke ORCID: 0000-0003-3190-0880, Gloeckner, Gernot, Guillou, Laure ORCID: 0000-0003-1032-7958 and John, Uwe (2014). Genomic Insights into Processes Driving the Infection of Alexandrium tamarense by the Parasitoid Amoebophrya sp. Eukaryot. Cell, 13 (11). S. 1439 - 1450. WASHINGTON: AMER SOC MICROBIOLOGY. ISSN 1535-9786

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Abstract

The regulatory circuits during infection of dinoflagellates by their parasites are largely unknown on the molecular level. Here we provide molecular insights into these infection dynamics. Alexandrium tamarense is one of the most prominent harmful algal bloom dinoflagellates. Its pathogen, the dinoflagellate parasitoid Amoebophrya sp., has been observed to infect and control the blooms of this species. We generated a data set of transcripts from three time points (0, 6, and 96 h) during the infection of this parasite-host system. Assembly of all transcript data from the parasitoid (> 900,000 reads/313 Mbp with 454/Roche next-generation sequencing [NGS]) yielded 14,455 contigs, to which we mapped the raw transcript reads of each time point of the infection cycle. We show that particular surface lectins are expressed at the beginning of the infection cycle which likely mediate the attachment to the host cell. In a later phase, signal transduction-related genes together with transmembrane transport and cytoskeleton proteins point to a high integration of processes involved in host recognition, adhesion, and invasion. At the final maturation stage, cell division-and proliferation-related genes were highly expressed, reflecting the fast cell growth and nuclear division of the parasitoid. Our molecular insights into dinoflagellate parasitoid interactions point to general mechanisms also known from other eukaryotic parasites, especially from the Alveolata. These similarities indicate the presence of fundamental processes of parasitoid infection that have remained stable throughout evolution within different phyla.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lu, Yameng UNSPECIFIED UNSPECIFIED UNSPECIFIED Wohlrab, Sylke UNSPECIFIED orcid.org/0000-0003-3190-0880 UNSPECIFIED Gloeckner, Gernot UNSPECIFIED UNSPECIFIED UNSPECIFIED Guillou, Laure UNSPECIFIED orcid.org/0000-0003-1032-7958 UNSPECIFIED John, Uwe UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-424634
DOI:	10.1128/EC.00139-14
Journal or Publication Title:	Eukaryot. Cell
Volume:	13
Number:	11
Page Range:	S. 1439 - 1450
Date:	2014
Publisher:	AMER SOC MICROBIOLOGY
Place of Publication:	WASHINGTON
ISSN:	1535-9786
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HEAT-SHOCK PROTEINS; HARMFUL ALGAL BLOOMS; TOXOPLASMA-GONDII; PERKINSUS-MARINUS; DINOFLAGELLATE AMOEBOPHRYA; GENUS ALEXANDRIUM; HOST; EVOLUTIONARY; METABOLISM; GENERATION Multiple languages Microbiology; Mycology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42463