Universität zu Köln

Holzinger, Andreas ORCID: 0000-0002-7745-3978, Kaplan, Franziska, Blaas, Kathrin, Zechmann, Bernd ORCID: 0000-0002-7702-2588, Komsic-Buchmann, Karin and Becker, Burkhard ORCID: 0000-0002-7965-1396 (2014). Transcriptomics of Desiccation Tolerance in the Streptophyte Green Alga Klebsormidium Reveal a Land Plant-Like Defense Reaction. PLoS One, 9 (10). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

Water loss has significant effects on physiological performance and survival rates of algae. However, despite the prominent presence of aeroterrestrial algae in terrestrial habitats, hardly anything is known about the molecular events that allow aeroterrestrial algae to survive harsh environmental conditions. We analyzed the transcriptome and physiology of a strain of the alpine aeroterrestrial alga Klebsormidium crenulatum under control and strong desiccation-stress conditions. Principal Findings: For comparison we first established a reference transcriptome. The high-coverage reference transcriptome includes about 24,183 sequences (1.5 million reads, 636 million bases). The reference transcriptome encodes for all major pathways (energy, carbohydrates, lipids, amino acids, sugars), nearly all deduced pathways are complete or missing only a few transcripts. Upon strong desiccation, more than 7000 transcripts showed changes in their expression levels. Most of the highest up-regulated transcripts do not show similarity to known viridiplant proteins, suggesting the existence of some genus-or species-specific responses to desiccation. In addition, we observed the up-regulation of many transcripts involved in desiccation tolerance in plants (e.g. proteins similar to those that are abundant in late embryogenesis (LEA), or proteins involved in early response to desiccation ERD), and enzymes involved in the biosynthesis of the raffinose family of oligosaccharides (RFO) known to act as osmolytes). Major physiological shifts are the up-regulation of transcripts for photosynthesis, energy production, and reactive oxygen species (ROS) metabolism, which is supported by elevated cellular glutathione content as revealed by immunoelectron microscopy as well as an increase in total antiradical power. However, the effective quantum yield of Photosystem II and CO2 fixation decreased sharply under the applied desiccation stress. In contrast, transcripts for cell integrative functions such as cell division, DNA replication, cofactor biosynthesis, and amino acid biosynthesis were down-regulated. Significance: This is the first study investigating the desiccation transcriptome of a streptophyte green alga. Our results indicate that the cellular response is similar to embryophytes, suggesting that embryophytes inherited a basic cellular desiccation tolerance from their streptophyte predecessors.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Holzinger, Andreas UNSPECIFIED orcid.org/0000-0002-7745-3978 UNSPECIFIED Kaplan, Franziska UNSPECIFIED UNSPECIFIED UNSPECIFIED Blaas, Kathrin UNSPECIFIED UNSPECIFIED UNSPECIFIED Zechmann, Bernd UNSPECIFIED orcid.org/0000-0002-7702-2588 UNSPECIFIED Komsic-Buchmann, Karin UNSPECIFIED UNSPECIFIED UNSPECIFIED Becker, Burkhard UNSPECIFIED orcid.org/0000-0002-7965-1396 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-425571
DOI:	10.1371/journal.pone.0110630
Journal or Publication Title:	PLoS One
Volume:	9
Number:	10
Date:	2014
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ALPINE SOIL CRUST; CRATEROSTIGMA-PLANTAGINEUM; STRESS; ANTIOXIDANTS; DEHYDRATION; CHLOROPHYTA; TEMPERATURE; CULTURE; LICHEN; ORIGIN Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/42557