Universität zu Köln

Sadler, Thomas, Kuster, Christian and von Elert, Eric ORCID: 0000-0001-7758-716X (2014). Seasonal dynamics of chemotypes in a freshwater phytoplankton community - A metabolomic approach. Harmful Algae, 39. S. 102 - 112. AMSTERDAM: ELSEVIER SCIENCE BV. ISSN 1878-1470

Full text not available from this repository.

Abstract

Cyanobacteria are known to produce a huge variety of secondary metabolites. Many of these metabolites are toxic to zooplankton, fish, birds and mammals. Therefore, the toxicity of cyanobacterial blooms is strongly dependent on the cyanobacterial strain composition. These strains produce distinct bouquets of secondary metabolites, and thus constitute different chemotypes. Some of the cyanobacterial metabolites are potent inhibitors of gut proteases of the filter-feeder Daphnia. Here, we investigate the seasonal dynamics of secondary metabolites in a phytoplankton community from a hypertrophic pond, making use of a new metabolomic approach. Using liquid chromatography coupled with high-resolution mass spectrometry (LCMS), we obtained mass spectra of phytoplankton samples taken on different dates throughout the summer season. By applying multivariate statistics, we combined these data with the protease inhibition capacity of the same samples. This led to the identification of metabolites with cyanobacterial origin and as well of distinct cyanobacterial chemotypes being dominant on different dates. The protease inhibition capacity varied strongly with season, and only one out of 73 known cyanobacterial protease inhibitors could be confirmed in the natural samples. Instead, several so far unknown, putative protease inhibitors were detected. In conclusion, the creation of time series of mass spectral data of a natural phytoplankton community proved to be useful for elucidating seasonal chemotype succession in a cyanobacterial community. Additionally, correlating mass spectral data with a biological assay provides a promising tool for facilitating the search for new harmful metabolites prior to structure elucidation. (C) 2014 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sadler, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuster, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED von Elert, Eric UNSPECIFIED orcid.org/0000-0001-7758-716X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-426334
DOI:	10.1016/j.hal.2014.07.006
Journal or Publication Title:	Harmful Algae
Volume:	39
Page Range:	S. 102 - 112
Date:	2014
Publisher:	ELSEVIER SCIENCE BV
Place of Publication:	AMSTERDAM
ISSN:	1878-1470
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CYANOBACTERIUM MICROCYSTIS-AERUGINOSA; PROTEASE INHIBITORS; DAPHNIA-MAGNA; CHYMOTRYPSIN INHIBITOR; TOXIN PRODUCTION; PLANKTOTHRIX-RUBESCENS; HARMFUL CYANOBACTERIA; OSCILLATORIA-AGARDHII; SECONDARY METABOLITES; POPULATION-GROWTH Multiple languages Marine & Freshwater Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42633