Universität zu Köln

Kunz, Hans-Henning, Zamani-Nour, Shirin, Haeusler, Rainer E., Ludewig, Katja, Schroeder, Julian I., Malinova, Irina, Fettke, Joerg ORCID: 0000-0003-0137-7611, Fluegge, Ulf-Ingo and Gierth, Markus (2014). Loss of Cytosolic Phosphoglucose Isomerase Affects Carbohydrate Metabolism in Leaves and Is Essential for Fertility of Arabidopsis. Plant Physiol., 166 (2). S. 753 - 778. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1532-2548

Full text not available from this repository.

Abstract

Carbohydrate metabolism in plants is tightly linked to photosynthesis and is essential for energy and carbon skeleton supply of the entire organism. Thus, the hexose phosphate pools of the cytosol and the chloroplast represent important metabolic resources that are maintained through action of phosphoglucose isomerase (PGI) and phosphoglucose mutase interconverting glucose 6-phosphate, fructose 6-phosphate, and glucose 1-phosphate. Here, we investigated the impact of disrupted cytosolic PGI (cPGI) function on plant viability and metabolism. Overexpressing an artificial microRNA targeted against cPGI (amiR-cpgi) resulted in adult plants with vegetative tissue essentially free of cPGI activity. These plants displayed diminished growth compared with the wild type and accumulated excess starch in chloroplasts but maintained low sucrose content in leaves at the end of the night. Moreover, amiR-cpgi plants exhibited increased nonphotochemical chlorophyll a quenching during photosynthesis. In contrast to amiR-cpgi plants, viable transfer DNA insertion mutants disrupted in cPGI function could only be identified as heterozygous individuals. However, homozygous transfer DNA insertion mutants could be isolated among plants ectopically expressing cPGI. Intriguingly, these plants were only fertile when expression was driven by the ubiquitin10 promoter but sterile when the seed-specific unknown seed protein promoter or the Cauliflower mosaic virus 35S promoter were employed. These data show that metabolism is apparently able to compensate for missing cPGI activity in adult amiR-cpgi plants and indicate an essential function for cPGI in plant reproduction. Moreover, our data suggest a feedback regulation in amiR-cpgi plants that fine-tunes cytosolic sucrose metabolism with plastidic starch turnover.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kunz, Hans-Henning UNSPECIFIED UNSPECIFIED UNSPECIFIED Zamani-Nour, Shirin UNSPECIFIED UNSPECIFIED UNSPECIFIED Haeusler, Rainer E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ludewig, Katja UNSPECIFIED UNSPECIFIED UNSPECIFIED Schroeder, Julian I. UNSPECIFIED UNSPECIFIED UNSPECIFIED Malinova, Irina UNSPECIFIED UNSPECIFIED UNSPECIFIED Fettke, Joerg UNSPECIFIED orcid.org/0000-0003-0137-7611 UNSPECIFIED Fluegge, Ulf-Ingo UNSPECIFIED UNSPECIFIED UNSPECIFIED Gierth, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-426399
DOI:	10.1104/pp.114.241091
Journal or Publication Title:	Plant Physiol.
Volume:	166
Number:	2
Page Range:	S. 753 - 778
Date:	2014
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	1532-2548
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TRIOSE-PHOSPHATE/PHOSPHATE TRANSLOCATOR; PLASTIDIC GLUCOSE TRANSLOCATOR; CHLOROPHYLL FLUORESCENCE; STARCH DEGRADATION; MALTOSE METABOLISM; GENE-EXPRESSION; ENZYME-ACTIVITY; THALIANA L; SUBCELLULAR-LOCALIZATION; ARTIFICIAL MICRORNAS Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42639