Universität zu Köln

Ronnberg, Elin ORCID: 0000-0002-4628-9968, Johnzon, Carl-Fredrik, Calounova, Gabriela, Faroldi, Gianni Garcia, Grujic, Mirjana, Hartmann, Karin ORCID: 0000-0002-4595-8226, Roers, Axel, Guss, Bengt ORCID: 0000-0002-7044-0701, Lundequist, Anders and Pejler, Gunnar (2014). Mast cells are activated by Staphylococcus aureus in vitro but do not influence the outcome of intraperitoneal S. aureus infection in vivo. Immunology, 143 (2). S. 155 - 164. HOBOKEN: WILEY. ISSN 1365-2567

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Abstract

Staphylococcus aureus is a major pathogen that can cause a broad spectrum of serious infections including skin infections, pneumonia and sepsis. Peritoneal mast cells have been implicated in the host response towards various bacterial insults and to provide mechanistic insight into the role of mast cells in intraperitoneal bacterial infection we here studied the global effects of S. aureus on mast cell gene expression. After co-culture of peritoneal mast cells with live S. aureus we found by gene array analysis that they up-regulate a number of genes. Many of these corresponded to pro-inflammatory cytokines, including interleukin-3, interleukin-13 and tumour necrosis factor-a. The cytokine induction in response to S. aureus was confirmed by ELISA. To study the role of peritoneal mast cells during in vivo infection with S. aureus we used newly developed Mcpt5-Cre(+) x R-DTA mice in which mast cell deficiency is independent of c-Kit. This is in contrast to previous studies in which an impact of mast cells on bacterial infection has been proposed based on the use of mice whose mast cell deficiency is a consequence of defective c-Kit signalling. Staphylococcus aureus was injected intraperitoneally into mast-cell-deficient Mcpt5-Cre(+) x R-DTA mice using littermate mast-cell-sufficient mice as controls. We did not observe any difference between mast-cell-deficient and control mice with regard to weight loss, bacterial clearance, inflammation or cytokine production. We conclude that, despite peritoneal mast cells being activated by S. aureus in vitro, they do not influence the in vivo manifestations of intraperitoneal S. aureus infection.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ronnberg, Elin UNSPECIFIED orcid.org/0000-0002-4628-9968 UNSPECIFIED Johnzon, Carl-Fredrik UNSPECIFIED UNSPECIFIED UNSPECIFIED Calounova, Gabriela UNSPECIFIED UNSPECIFIED UNSPECIFIED Faroldi, Gianni Garcia UNSPECIFIED UNSPECIFIED UNSPECIFIED Grujic, Mirjana UNSPECIFIED UNSPECIFIED UNSPECIFIED Hartmann, Karin UNSPECIFIED orcid.org/0000-0002-4595-8226 UNSPECIFIED Roers, Axel UNSPECIFIED UNSPECIFIED UNSPECIFIED Guss, Bengt UNSPECIFIED orcid.org/0000-0002-7044-0701 UNSPECIFIED Lundequist, Anders UNSPECIFIED UNSPECIFIED UNSPECIFIED Pejler, Gunnar UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-427334
DOI:	10.1111/imm.12297
Journal or Publication Title:	Immunology
Volume:	143
Number:	2
Page Range:	S. 155 - 164
Date:	2014
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1365-2567
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BACTERIAL CLEARANCE; MODEL; MICE; TNF Multiple languages Immunology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42733