Universität zu Köln

Herwig, Martina C., Muenstermann, Kathrin, Klarmann-Schulz, Ute, Schlereth, Simona L., Heindl, Ludwig M., Loeffler, Karin U. and Mueller, Annette M. (2014). Expression of the lymphatic marker podoplanin (D2-40) in human fetal eyes. Exp. Eye Res., 127. S. 243 - 252. LONDON: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD. ISSN 1096-0007

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Abstract

During human ocular development, expression of proteins varies in different maturation stages. This study aims to characterize structures in human fetal eyes stained by the lymphatic marker podoplanin (D2-40) with emphasis on the stage of maturation and the presence of intraocular lymphatic structures. Formalin-fixed paraffin-embedded eyes from 40 human fetuses between 10 and 38 weeks of gestation (WoG) were investigated. Immunohistochemical stains were performed for D2-40, LYVE-1 as a secondary lymphatic marker, and CD34 as a control for endothelial reactivity. A semiquantitative analysis of antigen expression in different segments of the eye was performed by light microscopy. The intensity of antigen expression was graded with a score ranging from 0 to 3. Podoplanin expression was found with a variable intensity in 97.5% of the eyes, in particular in lymphatic vessels of the conjunctiva (n = 26), conjunctival and corneal epithelium (n = 33), corneal endothelium (n = 4), trabecular meshwork (n = 28), and optic nerve sheaths (n = 23). A slight, equivocal staining reaction was noted in the choroid (n = 14). There was a correlation of antigen reactivity and the gestational age for corneal endothelial reactivity in earlier gestational stages (p = 0.003) and trabecular meshwork in older eyes (p = 0.031). D2-40 positive Muller cells were detected in two eyes >= 32 WoG. Thus, aside from conjunctival lymphatic vessels, podoplanin was expressed in several structures of the human fetal eye and the ocular adnexae at different gestational stages. Podoplanin positive structures were also found in the choroid and the chamber angle. However, lymphatic vessels or its progenitors could not be unequivocally identified in intraocular structures during 10-38 weeks of gestation. There is no evidence from our data that transient intraocular lymphactics develop in the fetal eye between 10 and 38 weeks of gestation. (C) 2014 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Herwig, Martina C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Muenstermann, Kathrin UNSPECIFIED UNSPECIFIED UNSPECIFIED Klarmann-Schulz, Ute UNSPECIFIED UNSPECIFIED UNSPECIFIED Schlereth, Simona L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Heindl, Ludwig M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Loeffler, Karin U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Annette M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-427482
DOI:	10.1016/j.exer.2014.07.026
Journal or Publication Title:	Exp. Eye Res.
Volume:	127
Page Range:	S. 243 - 252
Date:	2014
Publisher:	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Place of Publication:	LONDON
ISSN:	1096-0007
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language OPTIC-NERVE SHEATH; CILIARY BODY; HUMAN CORNEA; VESSELS; LYMPHANGIOGENESIS; IMMUNOREACTIVITY; ENDOTHELIUM; ANTIBODY; IMMUNOHISTOCHEMISTRY; ANGIOGENESIS Multiple languages Ophthalmology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42748