Universität zu Köln

Kabacam, Gokhan ORCID: 0000-0002-2099-9697, Wedemeyer, Heiner, Savas, Berna ORCID: 0000-0003-3971-1419, Keskin, Onur, Dalekos, George, Tabak, Fehmi, Idilman, Ramazan, Erhardt, Andreas, Yalcin, Kendal, Bozdayi, Mithat A., Bozkaya, Hakan, Manns, Michael, Dienes, Hans and Yurdaydin, Cihan (2014). Role of immunohistochemistry for hepatitis D and hepatitis B virus in hepatitis delta. Liver Int., 34 (8). S. 1207 - 1216. HOBOKEN: WILEY. ISSN 1478-3231

Full text not available from this repository.

Abstract

Background & Aims: Immunohistochemical assessment of liver tissue in chronic delta hepatitis (CDH) is underinvestigated. Aim of the study was (i) to assess variables associated with hepatitis D antigen (HDAg), hepatitis B surface antigen (HBsAg) and hepatitis B core antigen (HBcAg) staining in the liver. Methods: Demographic, biochemical and virologic data collected from the HIDIT 1 study were used. HBsAg, HBcAg and HDAg immunohistochemical (IHC) staining was semiquantitatively assessed. Results: Hepatitis D antigen immunohistochemical staining displayed positive correlations with age and alanine aminotransferase (ALT) and negative correlations with serum HBsAg (P = 0.01 for all). HBsAg IHC displayed a negative correlation with gamma glutamyl transferase and positive correlations with serum HBV DNA, serum HBsAg levels and HBeAg serology (P < 0.001, P = 0.02 and P = 0.007 respectively). HBcAg staining was mainly nuclear and displayed negative correlations with serum HBsAg and histologic activity (P = 0.002 and P = 0.02 respectively). Pegylated IFN based treatment led to a decline of all IHC markers, however, these markers had no impact on treatment outcome. Conclusions: These data suggest an association of liver injury with HDAg expression in CDH whereas the negative correlation between HBcAg expression and liver injury and the overall nuclear localization of HBcAg suggest that HBcAg does not contribute to liver injury in CDH. HDV cases with high level of HBV replication, high serum HBsAg levels, HBeAg positivity, that are probably in the earlier stages of disease (low gamma-glutamyl transferase), had a more intense HBsAg staining profile. Overall, the data enforce the importance of HDAg and HBsAg in different phases of CDH infection.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kabacam, Gokhan UNSPECIFIED orcid.org/0000-0002-2099-9697 UNSPECIFIED Wedemeyer, Heiner UNSPECIFIED UNSPECIFIED UNSPECIFIED Savas, Berna UNSPECIFIED orcid.org/0000-0003-3971-1419 UNSPECIFIED Keskin, Onur UNSPECIFIED UNSPECIFIED UNSPECIFIED Dalekos, George UNSPECIFIED UNSPECIFIED UNSPECIFIED Tabak, Fehmi UNSPECIFIED UNSPECIFIED UNSPECIFIED Idilman, Ramazan UNSPECIFIED UNSPECIFIED UNSPECIFIED Erhardt, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Yalcin, Kendal UNSPECIFIED UNSPECIFIED UNSPECIFIED Bozdayi, Mithat A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bozkaya, Hakan UNSPECIFIED UNSPECIFIED UNSPECIFIED Manns, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Dienes, Hans UNSPECIFIED UNSPECIFIED UNSPECIFIED Yurdaydin, Cihan UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-429826
DOI:	10.1111/liv.12376
Journal or Publication Title:	Liver Int.
Volume:	34
Number:	8
Page Range:	S. 1207 - 1216
Date:	2014
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1478-3231
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language INTRAHEPATIC EXPRESSION; SURFACE-ANTIGEN; CORE ANTIGEN; LIVER; DISEASE; MARKERS; NUCLEAR; SERUM Multiple languages Gastroenterology & Hepatology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/42982