Universität zu Köln

Eifinger, Frank, Lubomirov, Lubomir T., Dercks, Elena, Genchev, Borislav, Roth, Bernhard, Neiss, Wolfram F., Pfitzer, Gabriele and Schroeter, Mechthild M. (2014). Neonatal mouse ileum: functional properties and protein composition of the contractile machinery. Pediatr. Res., 76 (3). S. 252 - 261. NEW YORK: NATURE PUBLISHING GROUP. ISSN 1530-0447

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Abstract

BACKGROUND: Immature motility of the ileum may contribute to life-threatening diseases. Little is known about the normal biomechanics of the neonatal ileum in relation to the protein composition of its contractile machinery. METHODS: We analyzed the tissue architecture, the biomechanics in intact and beta-escin-permeabilized preparations, and the protein composition in neonatal (PO) and adult murine ileum. RESULTS: Muscle thickness of the PO ileum was -50% of the adult ileum and passive compliance was higher. Carbachol- and KCI-elicited contractions were tonic rather than phasic as in the adult. Ca2+ sensitivity was higher and relaxation rate was slower in beta-escin-permeabilized PO compared with adult ileum. The expression level of beta-actin relative to alpha-actin was higher, and those of total actin, myosin, myosin light chain kinase, the catalytic subunit of myosin phosphatase and telokin were lower compared with the adult. The expression level of MYPT1 was similar, but PO ileum expressed only the M133; the adult ileum also expressed the M130 isoform. CONCLUSION: The mechanical features and protein composition of the PO ileum are similar to those of adult tonic smooth muscles. We propose that this is highly adaptive during fetal life allowing the small intestine to act predominantly as a container.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Eifinger, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED Lubomirov, Lubomir T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Dercks, Elena UNSPECIFIED UNSPECIFIED UNSPECIFIED Genchev, Borislav UNSPECIFIED UNSPECIFIED UNSPECIFIED Roth, Bernhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Neiss, Wolfram F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Pfitzer, Gabriele UNSPECIFIED UNSPECIFIED UNSPECIFIED Schroeter, Mechthild M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-430750
DOI:	10.1038/pr.2014.91
Journal or Publication Title:	Pediatr. Res.
Volume:	76
Number:	3
Page Range:	S. 252 - 261
Date:	2014
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	NEW YORK
ISSN:	1530-0447
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SMOOTH-MUSCLE CONTRACTION; LIGHT-CHAIN PHOSPHORYLATION; ENTERIC NERVOUS-SYSTEM; MYOSIN PHOSPHATASE; INTERSTITIAL-CELLS; DEVELOPMENTAL-CHANGES; INTESTINAL MOTILITY; ACTOMYOSIN CONTENT; CA2+ SENSITIVITY; FETAL MICE Multiple languages Pediatrics Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43075