Universität zu Köln

Frerigmann, Henning ORCID: 0000-0002-7067-2721, Berger, Bettina ORCID: 0000-0003-1195-4478 and Gigolashvili, Tamara ORCID: 0000-0002-0416-4796 (2014). bHLH05 Is an Interaction Partner of MYB51 and a Novel Regulator of Glucosinolate Biosynthesis in Arabidopsis. Plant Physiol., 166 (1). S. 349 - 370. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1532-2548

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Abstract

By means of yeast (Saccharomyces cerevisiae) two-hybrid screening, we identified basic helix-loop-helix transcription factor05 (bHLH05) as an interacting partner of MYB51, the key regulator of indolic glucosinolates (GSLs) in Arabidopsis (Arabidopsis thaliana). Furthermore, we show that bHLH04, bHLH05, and bHLH06/MYC2 also interact with other R2R3-MYBs regulating GSL biosynthesis. Analysis of bhlh loss-of-function mutants revealed that the single bhlh mutants retained GSL levels that were similar to those in wild-type plants, whereas the triple bhlh04/05/06 mutant was depleted in the production of GSL. Unlike bhlh04/06 and bhlh05/06 mutants, the double bhlh04/05 mutant was strongly affected in the production of GSL, pointing to a special role of bHLH04 and bHLH05 in the control of GSL levels in the absence of jasmonic acid. The combination of two specific gain-of-function alleles of MYB and bHLH proteins had an additive effect on GSL levels, as demonstrated by the analysis of the double MYB34-1D bHLH05D94N mutant, which produces 20-fold more indolic GSLs than bHLH05D94N and ecotype Columbia-0 of Arabidopsis. The amino acid substitution D94N in bHLH05D94N negatively affects the interaction with JASMONATE-ZIM DOMAIN protein, thereby resulting in constitutive activation of bHLH05 and mimicking jasmonic acid treatment. Our study revealed the bHLH04, bHLH05, and bHLH06/MYC2 factors as novel regulators of GSL biosynthesis in Arabidopsis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Frerigmann, Henning UNSPECIFIED orcid.org/0000-0002-7067-2721 UNSPECIFIED Berger, Bettina UNSPECIFIED orcid.org/0000-0003-1195-4478 UNSPECIFIED Gigolashvili, Tamara UNSPECIFIED orcid.org/0000-0002-0416-4796 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-431387
DOI:	10.1104/pp.114.240887
Journal or Publication Title:	Plant Physiol.
Volume:	166
Number:	1
Page Range:	S. 349 - 370
Date:	2014
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	1532-2548
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HELIX TRANSCRIPTION FACTORS; MEDIATOR COMPLEX; OILSEED RAPE; ANTHOCYANIN BIOSYNTHESIS; JASMONATE RESPONSES; PROMOTER INTERACTIONS; COORDINATED CONTROL; SIGNALING PATHWAYS; GENE-TRANSCRIPTION; METHYL JASMONATE Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/43138