Universität zu Köln

Koprivova, Anna, Harper, Andrea L., Trick, Martin, Bancroft, Ian ORCID: 0000-0001-7707-1171 and Kopriva, Stanislav ORCID: 0000-0002-7416-6551 (2014). Dissection of the Control of Anion Homeostasis by Associative Transcriptomics in Brassica napus. Plant Physiol., 166 (1). S. 442 - 452. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1532-2548

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Abstract

To assess the variation in nutrient homeostasis in oilseed rape and to identify the genes responsible for this variation, we determined foliar anion levels in a diversity panel of Brassica napus accessions, 84 of which had been genotyped previously using messenger RNA sequencing. We applied associative transcriptomics to identify sequence polymorphisms linked to variation in nitrate, phosphate, or sulfate in these accessions. The analysis identified several hundred significant associations for each anion. Using functional annotation of Arabidopsis (Arabidopsis thaliana) homologs and available microarray data, we identified 60 candidate genes for controlling variation in the anion contents. To verify that these genes function in the control of nutrient homeostasis, we obtained Arabidopsis transfer DNA insertion lines for these candidates and tested them for the accumulation of nitrate, phosphate, and sulfate. Fourteen lines differed significantly in levels of the corresponding anions. Several of these genes have been shown previously to affect the accumulation of the corresponding anions in Arabidopsis mutants. These results thus confirm the power of associative transcriptomics in dissection of the genetic control of complex traits and present a set of candidate genes for use in the improvement of efficiency of B. napus mineral nutrition.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Koprivova, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Harper, Andrea L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Trick, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Bancroft, Ian UNSPECIFIED orcid.org/0000-0001-7707-1171 UNSPECIFIED Kopriva, Stanislav UNSPECIFIED orcid.org/0000-0002-7416-6551 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-431390
DOI:	10.1104/pp.114.239947
Journal or Publication Title:	Plant Physiol.
Volume:	166
Number:	1
Page Range:	S. 442 - 452
Date:	2014
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	1532-2548
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ADENOSINE 5-PHOSPHOSULFATE REDUCTASE; GENOME-WIDE ASSOCIATION; NITROGEN USE EFFICIENCY; TRAIT LOCI ANALYSIS; ARABIDOPSIS-THALIANA; PHOSPHATE STARVATION; NATURAL VARIATION; UBIQUITIN LIGASE; PLASMA-MEMBRANE; GENE-EXPRESSION Multiple languages Plant Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/43139