Universität zu Köln

Balcerowicz, Martin ORCID: 0000-0001-9462-4436, Ranjan, Aashish ORCID: 0000-0003-3447-4442, Rupprecht, Laura, Fiene, Gabriele and Hoecker, Ute ORCID: 0000-0002-5636-9777 (2014). Auxin represses stomatal development in dark-grown seedlings via Aux/IAA proteins. Development, 141 (16). S. 3165 - 3177. CAMBRIDGE: COMPANY BIOLOGISTS LTD. ISSN 1477-9129

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Abstract

Stomatal development is tightly regulated through internal and external factors that are integrated by a complex signalling network. Light represents an external factor that strongly promotes stomata formation. Here, we show that auxin-resistant aux/iaa mutants, e.g. axr3-1, exhibit a de-repression of stomata differentiation in dark-grown seedlings. The higher stomatal index in dark-grown axr3-1 mutants when compared with the wild type is due to increased cell division in the stomatal lineage. Excessive stomata in dark-grown seedlings were also observed in mutants defective in auxin biosynthesis or auxin perception and in seedlings treated with the polar auxin transport inhibitor NPA. Consistent with these findings, exogenous auxin repressed stomata formation in light-grown seedlings. Taken together, these results indicate that auxin is a negative regulator of stomatal development in dark-grown seedlings. Epistasis analysis revealed that axr3-1 acts genetically upstream of the bHLH transcription factors SPCH, MUTE and FAMA, as well as the YDA MAP kinase cascade, but in parallel with the repressor of photomorphogenesis COP1 and the receptor-like protein TMM. The effect of exogenous auxin required the ER family of leucine-rich repeat receptor-like kinases, suggesting that auxin acts at least in part through the ER family. Expression of axr3-1 in the stomatal lineage was insufficient to alter the stomatal index, implying that cell-cell communication is necessary to mediate the effect of auxin. In summary, our results show that auxin signalling contributes to the suppression of stomatal differentiation observed in dark-grown seedlings.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Balcerowicz, Martin UNSPECIFIED orcid.org/0000-0001-9462-4436 UNSPECIFIED Ranjan, Aashish UNSPECIFIED orcid.org/0000-0003-3447-4442 UNSPECIFIED Rupprecht, Laura UNSPECIFIED UNSPECIFIED UNSPECIFIED Fiene, Gabriele UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoecker, Ute UNSPECIFIED orcid.org/0000-0002-5636-9777 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-432506
DOI:	10.1242/dev.109181
Journal or Publication Title:	Development
Volume:	141
Number:	16
Page Range:	S. 3165 - 3177
Date:	2014
Publisher:	COMPANY BIOLOGISTS LTD
Place of Publication:	CAMBRIDGE
ISSN:	1477-9129
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ARABIDOPSIS-THALIANA; SHADE-AVOIDANCE; PHYTOCHROME B; PLANT DEVELOPMENT; MAPK; KINASES; FAMILY; ACTIVATION; EXPRESSION; GENE Multiple languages Developmental Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/43250