Universität zu Köln

Deng, Li ORCID: 0000-0003-0225-0663, Krauss, Steffen, Feichtmayer, Judith, Hofmann, Roland, Arndt, Hartmut ORCID: 0000-0003-2811-3595 and Griebler, Christian ORCID: 0000-0002-8602-581X (2014). Grazing of heterotrophic flagellates on viruses is driven by feeding behaviour. Environ. Microbiol. Rep., 6 (4). S. 325 - 331. HOBOKEN: WILEY-BLACKWELL. ISSN 1758-2229

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Abstract

The trophic interactions between viruses, bacteria and protists play a crucial role in structuring microbial communities and regulating nutrient and organic matter flux. Here, we show that the impact on viral density by heterotrophic flagellates is related to their feeding behaviour (feeding on sedimented particles Thaumatomonas coloniensis, filter feeding of suspended particles - Salpingoeca sp., and actively searching raptorial feeding - Goniomonas truncata). Phage MS2 was co-incubated with flagellates and the natural bacterial and viral community originating from the same groundwater habitats where the flagellates were isolated. Three complementary assays, i.e. flow cytometry, qPCR and plaque assay, were used for enumeration of total viruses, total MS2 phages, and free and infectious MS2, respectively, to provide insights into the grazing mechanisms of the flagellates on viruses. Phage MS2 was actively removed by the suspension feeders T. coloniensis and Salpingoeca sp. in contrast with the actively raptoriale grazer G. truncata. The decline of viral titre was demonstrated to be caused by ingestion rather than random absorption by both qPCR and locating protein fluorescently labelled MS2 inside the flagellates. Further, we indicate that phages can be used as a minor carbon source for flagellates. Collectively, these data demonstrate that eliminating viruses can be an important function of protists in microbial food webs, carbon cycling and potentially water quality control.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Deng, Li UNSPECIFIED orcid.org/0000-0003-0225-0663 UNSPECIFIED Krauss, Steffen UNSPECIFIED UNSPECIFIED UNSPECIFIED Feichtmayer, Judith UNSPECIFIED UNSPECIFIED UNSPECIFIED Hofmann, Roland UNSPECIFIED UNSPECIFIED UNSPECIFIED Arndt, Hartmut UNSPECIFIED orcid.org/0000-0003-2811-3595 UNSPECIFIED Griebler, Christian UNSPECIFIED orcid.org/0000-0002-8602-581X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-433247
DOI:	10.1111/1758-2229.12119
Journal or Publication Title:	Environ. Microbiol. Rep.
Volume:	6
Number:	4
Page Range:	S. 325 - 331
Date:	2014
Publisher:	WILEY-BLACKWELL
Place of Publication:	HOBOKEN
ISSN:	1758-2229
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MARINE VIRUSES; TETRAHYMENA-THERMOPHILA; AQUATIC ENVIRONMENTS; CLAY-MINERALS; INACTIVATION; WATER; BACTERIOPHAGES; BACTERIA; PARTICLES; SURVIVAL Multiple languages Environmental Sciences; Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43324