Universität zu Köln

Theissen, Jessica, Oberthuer, Andre, Hombach, Anja, Volland, Ruth, Hertwig, Falk ORCID: 0000-0003-4784-6516, Fischer, Matthias, Spitz, Ruediger, Zapatka, Marc ORCID: 0000-0001-8287-5967, Brors, Benedikt ORCID: 0000-0001-5940-3101, Ortmann, Monika, Simon, Thorsten, Hero, Barbara and Berthold, Frank (2014). Chromosome 17/17q gain and unaltered profiles in high resolution array-CGH are prognostically informative in neuroblastoma. Gene Chromosomes Cancer, 53 (8). S. 639 - 650. HOBOKEN: WILEY. ISSN 1098-2264

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Abstract

The prognostic relevance of chromosome 17 gain in neuroblastoma is still discussed. This investigation specifies the frequency, type, size, and transcriptional relevance in a large patient cohort. Primary tumor material of 202 patients was analyzed using high-resolution oligonucleotide array-based comparative genomic hybridization (aCGH) and correlated with clinical and survival data. A subset (n=145) was correlated for differentially expressed genes (DEG) by microarray analysis. Chromosome 17 aCGH analysis showed numerical gain in 94/202 patients (47%), partial gain in 93/202 patients (46%), and no gain in 15/202 patients (7%). The frequency of partial gain was higher in stage 4 neuroblastoma (stage 1 15%; stage 2 12%; stage 3 16%; stage 4S 7%; and stage 4 50%). Overall survival (OS) was superior in patients with numerical gain compared with patients with partial gain or no gain (5-y-OS: 0.95 +/- 0.02 vs. 0.63 +/- 0.05 vs. 0.60 +/- 0.13; P<0.001). Gene expression analysis demonstrated 95/130 DEGs between tumors with numerical or partial chromosome/no gain. Only one DEG (CCKBR) was detected comparing tumors with partial gain and those with no gain. In patients with partial gain, the distribution of breakpoints did not correlate with stage and 11q status, but with MYCN amplification and 1p status. The best breakpoints in cases with partial 17q gain were at 42.5 Mb for event-free and 26.6 Mb for OS. Numerical gain of chromosome 17 is associated with a better prognosis than partial and no gain. The group of tumors with partial gain was similar to the group without gain with respect to stage distribution, outcome, and gene expression profile. (c) 2014 Wiley Periodicals, Inc.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Theissen, Jessica UNSPECIFIED UNSPECIFIED UNSPECIFIED Oberthuer, Andre UNSPECIFIED UNSPECIFIED UNSPECIFIED Hombach, Anja UNSPECIFIED UNSPECIFIED UNSPECIFIED Volland, Ruth UNSPECIFIED UNSPECIFIED UNSPECIFIED Hertwig, Falk UNSPECIFIED orcid.org/0000-0003-4784-6516 UNSPECIFIED Fischer, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Spitz, Ruediger UNSPECIFIED UNSPECIFIED UNSPECIFIED Zapatka, Marc UNSPECIFIED orcid.org/0000-0001-8287-5967 UNSPECIFIED Brors, Benedikt UNSPECIFIED orcid.org/0000-0001-5940-3101 UNSPECIFIED Ortmann, Monika UNSPECIFIED UNSPECIFIED UNSPECIFIED Simon, Thorsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Hero, Barbara UNSPECIFIED UNSPECIFIED UNSPECIFIED Berthold, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-433556
DOI:	10.1002/gcc.22174
Journal or Publication Title:	Gene Chromosomes Cancer
Volume:	53
Number:	8
Page Range:	S. 639 - 650
Date:	2014
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1098-2264
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXPRESSION-BASED CLASSIFICATION; 17Q TRANSLOCATION BREAKPOINTS; ARM 17Q; RISK GROUP; OUTCOME PREDICTION; GENE; MYCN; PROGNOSIS; 11Q; DELINEATION Multiple languages Oncology; Genetics & Heredity Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43355