Universität zu Köln

Behrens, Maik ORCID: 0000-0003-2082-8860, Frank, Oliver, Rawel, Harshadrai ORCID: 0000-0003-2768-1531, Ahuja, Gaurav, Potting, Christoph, Hofmann, Thomas, Meyerhof, Wolfgang and Korsching, Sigrun ORCID: 0000-0002-5450-172X (2014). ORA1, a Zebrafish Olfactory Receptor Ancestral to All Mammalian V1R Genes, Recognizes 4-Hydroxyphenylacetic Acid, a Putative Reproductive Pheromone. J. Biol. Chem., 289 (28). S. 19778 - 19789. BETHESDA: AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC. ISSN 1083-351X

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Abstract

The teleost v1r-related ora genes are a small, highly conserved olfactory receptor gene family of only six genes, whose direct orthologues can be identified in lineages as far as that of cartilaginous fish. However, no ligands for fish olfactory receptor class A related genes (ORA) had been uncovered so far. Here we have deorphanized the ORA1 receptor using heterologous expression and calcium imaging. We report that zebrafish ORA1 recognizes with high specificity and sensitivity 4-hydroxyphenylacetic acid. The carboxyl group of this compound is required in a particular distance from the aromatic ring, whereas the hydroxyl group in the para-position is not essential, but strongly enhances the binding efficacy. Low concentrations of 4-hydroxyphenylacetic acid elicit increases in oviposition frequency in zebrafish mating pairs. This effect is abolished by naris closure. We hypothesize that 4-hydroxyphenylacetic acid might function as a pheromone for reproductive behavior in zebrafish. ORA1 is ancestral to mammalian V1Rs, and its putative function as pheromone receptor is reminiscent of the role of several mammalian V1Rs as pheromone receptors.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Behrens, Maik UNSPECIFIED orcid.org/0000-0003-2082-8860 UNSPECIFIED Frank, Oliver UNSPECIFIED UNSPECIFIED UNSPECIFIED Rawel, Harshadrai UNSPECIFIED orcid.org/0000-0003-2768-1531 UNSPECIFIED Ahuja, Gaurav UNSPECIFIED UNSPECIFIED UNSPECIFIED Potting, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Hofmann, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Meyerhof, Wolfgang UNSPECIFIED UNSPECIFIED UNSPECIFIED Korsching, Sigrun UNSPECIFIED orcid.org/0000-0002-5450-172X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-433980
DOI:	10.1074/jbc.M114.573162
Journal or Publication Title:	J. Biol. Chem.
Volume:	289
Number:	28
Page Range:	S. 19778 - 19789
Date:	2014
Publisher:	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Place of Publication:	BETHESDA
ISSN:	1083-351X
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language VOMERONASAL RECEPTOR; TASTE RECEPTORS; BITTER TASTE; FUNCTIONAL EXPRESSION; SENSORY NEURONS; ODORANT; BULB; FAMILIES; COMBINATORIAL; SPECIFICITY Multiple languages Biochemistry & Molecular Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/43398