Universität zu Köln

Castaings, Loren ORCID: 0000-0001-6830-4527, Bergonzi, Sara, Albani, Maria C. ORCID: 0000-0002-8215-020X, Kemi, Ulla, Savolainen, Outi and Coupland, George ORCID: 0000-0001-6988-4172 (2014). Evolutionary conservation of cold-induced antisense RNAs of FLOWERING LOCUS C in Arabidopsis thaliana perennial relatives. Nat. Commun., 5. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

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Abstract

Antisense RNA (asRNA) COOLAIR is expressed at A. thaliana FLOWERING LOCUS C (FLC) in response to winter temperatures. Its contribution to cold-induced silencing of FLC was proposed but its functional and evolutionary significance remain unclear. Here we identify a highly conserved block containing the COOLAIR first exon and core promoter at the 3' end of several FLC orthologues. Furthermore, asRNAs related to COOLAIR are expressed at FLC loci in the perennials A. alpina and A. lyrata, although some splicing variants differ from A. thaliana. Study of the A. alpina orthologue, PERPETUAL FLOWERING 1 (PEP1), demonstrates that AaCOOLAIR is induced each winter of the perennial life cycle. Introduction of PEP1 into A. thaliana reveals that AaCOOLAIR cis-elements confer cold-inducibility in this heterologous species while the difference between PEP1 and FLC mRNA patterns depends on both cis-elements and species-specific trans-acting factors. Thus, expression of COOLAIR is highly conserved, supporting its importance in FLC regulation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Castaings, Loren UNSPECIFIED orcid.org/0000-0001-6830-4527 UNSPECIFIED Bergonzi, Sara UNSPECIFIED UNSPECIFIED UNSPECIFIED Albani, Maria C. UNSPECIFIED orcid.org/0000-0002-8215-020X UNSPECIFIED Kemi, Ulla UNSPECIFIED UNSPECIFIED UNSPECIFIED Savolainen, Outi UNSPECIFIED UNSPECIFIED UNSPECIFIED Coupland, George UNSPECIFIED orcid.org/0000-0001-6988-4172 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-434320
DOI:	10.1038/ncomms5457
Journal or Publication Title:	Nat. Commun.
Volume:	5
Date:	2014
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2041-1723
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language VERNALIZATION-INDUCED REPRESSION; EPIGENETIC MAINTENANCE; FUNCTIONAL ELEMENTS; REGULATORY ELEMENTS; SEQUENCE-ANALYSIS; NONCODING RNA; TIME CONTROL; HISTONE H3; FLC LOCUS; LYSINE 27 Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/43432