Universität zu Köln

Sewelam, Nasser, Jaspert, Nils, Van der Kelen, Katrien, Tognetti, Vanesa B., Schmitz, Jessica, Frerigmann, Henning ORCID: 0000-0002-7067-2721, Stahl, Elia, Zeier, Juergen, Van Breusegem, Frank ORCID: 0000-0002-3147-0860 and Maurino, Veronica G. (2014). Spatial H2O2 Signaling Specificity: H2O2 from Chloroplasts and Peroxisomes Modulates the Plant Transcriptome Differentially. Mol. Plant., 7 (7). S. 1191 - 1211. CAMBRIDGE: CELL PRESS. ISSN 1752-9867

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Abstract

Hydrogen peroxide (H2O2) operates as a signaling molecule in eukaryotes, but the specificity of its signaling capacities remains largely unrevealed. Here, we analyzed whether a moderate production of H2O2 from two different plant cellular compartments has divergent effects on the plant transcriptome. Arabidopsis thaliana overexpressing glycolate oxidase in the chloroplast (Fahnenstich et al., 2008; Balazadeh et al., 2012) and plants deficient in peroxisomal catalase (Queval et al., 2007; Inze et al., 2012) were grown under non-photorespiratory conditions and then transferred to photorespiratory conditions to foster the production of H2O2 in both organelles. We show that H2O2 originating in a specific organelle induces two types of responses: one that integrates signals independently from the subcellular site of H2O2 production and another that is dependent on the H2O2 production site. H2O2 produced in peroxisomes induces transcripts involved in protein repair responses, while H2O2 produced in chloroplasts induces early signaling responses, including transcription factors and biosynthetic genes involved in production of secondary signaling messengers. There is a significant bias towards the induction of genes involved in responses to wounding and pathogen attack by chloroplastic-produced H2O2, including indolic glucosinolates-, camalexin-, and stigmasterol-biosynthetic genes. These transcriptional (r)esponses were accompanied by the accumulation of 4-methoxy-indol-3-ylmethyl glucosinolate and stigmasterol.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sewelam, Nasser UNSPECIFIED UNSPECIFIED UNSPECIFIED Jaspert, Nils UNSPECIFIED UNSPECIFIED UNSPECIFIED Van der Kelen, Katrien UNSPECIFIED UNSPECIFIED UNSPECIFIED Tognetti, Vanesa B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmitz, Jessica UNSPECIFIED UNSPECIFIED UNSPECIFIED Frerigmann, Henning UNSPECIFIED orcid.org/0000-0002-7067-2721 UNSPECIFIED Stahl, Elia UNSPECIFIED UNSPECIFIED UNSPECIFIED Zeier, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Van Breusegem, Frank UNSPECIFIED orcid.org/0000-0002-3147-0860 UNSPECIFIED Maurino, Veronica G. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-434389
DOI:	10.1093/mp/ssu070
Journal or Publication Title:	Mol. Plant.
Volume:	7
Number:	7
Page Range:	S. 1191 - 1211
Date:	2014
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	1752-9867
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language INTRACELLULAR HYDROGEN-PEROXIDE; OXIDATIVE STRESS-RESPONSE; INDUCED GENE-EXPRESSION; SUBCELLULAR-LOCALIZATION; ARABIDOPSIS-THALIANA; CELL-DEATH; HIGH LIGHT; OXYGEN; REVEALS; ACID Multiple languages Biochemistry & Molecular Biology; Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43438