Universität zu Köln

Ranjan, Aashish ORCID: 0000-0003-3447-4442, Dickopf, Stephen, Ullrich, Kristian K., Rensing, Stefan A. and Hoecker, Ute (2014). Functional analysis of COP1 and SPA orthologs from Physcomitrella and rice during photomorphogenesis of transgenic Arabidopsis reveals distinct evolutionary conservation. BMC Plant Biol., 14. LONDON: BIOMED CENTRAL LTD. ISSN 1471-2229

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Abstract

Background: Plants have evolved light sensing mechanisms to optimally adapt their growth and development to the ambient light environment. The COP1/SPA complex is a key negative regulator of light signaling in the well-studied dicot Arabidopsis thaliana. COP1 and members of the four SPA proteins are part of an E3 ubiquitin ligase that acts in darkness to ubiquitinate several transcription factors involved in light responses, thereby targeting them for degradation by the proteasome. While COP1 is also found in humans, SPA proteins appear specific to plants. Here, we have functionally addressed evolutionary conservation of COP1 and SPA orthologs from the moss Physcomitrella, the monocot rice and the dicot Arabidopsis. Results: To this end, we analyzed the activities of COP1- and SPA-like proteins from Physcomitrella patens and rice when expressed in Arabidopsis. Expression of rice COP1 and Physcomitrella COP1 protein sequences predominantly complemented all phenotypic aspects of the viable, hypomorphic cop1-4 mutant and the null, seedling-lethal cop1-5 mutant of Arabidopsis: rice COP1 fully rescued the constitutive-photomorphogenesis phenotype in darkness and the leaf expansion defect of cop1 mutants, while it partially restored normal photoperiodic flowering in cop1. Physcomitrella COP1 partially restored normal seedling growth and flowering time, while it fully restored normal leaf expansion in the cop1 mutants. In contrast, expression of a SPA ortholog from Physcomitrella (PpSPAb) in Arabidopsis spa mutants did not rescue any facet of the spa mutant phenotype, suggesting that the PpSPAb protein is not functionally conserved or that the Arabidopsis function evolved after the split of mosses and seed plants. The SPA1 ortholog from rice (OsSPA1) rescued the spa mutant phenotype in dark-grown seedlings, but did not complement any spa mutant phenotype in light-grown seedlings or in adult plants. Conclusion: Our results show that COP1 protein sequences from Physcomitrella, rice and Arabidopsis have been functionally conserved during evolution, while the SPA proteins showed considerable functional divergence. This may - at least in part - reflect the fact that COP1 is a single copy gene in seed plants, while SPA proteins are encoded by a small gene family of two to four members with possibly sub-or neofunctionalized tasks.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ranjan, Aashish UNSPECIFIED orcid.org/0000-0003-3447-4442 UNSPECIFIED Dickopf, Stephen UNSPECIFIED UNSPECIFIED UNSPECIFIED Ullrich, Kristian K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Rensing, Stefan A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoecker, Ute UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-434663
DOI:	10.1186/1471-2229-14-178
Journal or Publication Title:	BMC Plant Biol.
Volume:	14
Date:	2014
Publisher:	BIOMED CENTRAL LTD
Place of Publication:	LONDON
ISSN:	1471-2229
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MULTIPLE SEQUENCE ALIGNMENT; LIGHT-DEPENDENT INTERACTION; BLUE-LIGHT; SIGNAL-TRANSDUCTION; TRANSCRIPTION FACTORS; FLOWERING TIME; CRYPTOCHROME 1; RED-LIGHT; PHYTOCHROME; PROTEIN Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43466