Universität zu Köln

Willenborg, Sebastian, Eckes, Beate, Brinckmann, Juergen, Krieg, Thomas, Waisman, Ari ORCID: 0000-0003-4304-8234, Hartmann, Karin ORCID: 0000-0002-4595-8226, Roers, Axel and Eming, Sabine A. (2014). Genetic Ablation of Mast Cells Redefines the Role of Mast Cells in Skin Wound Healing and Bleomycin-Induced Fibrosis. J. Invest. Dermatol., 134 (7). S. 2005 - 2016. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1523-1747

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Abstract

Conclusive evidence for the impact of mast cells (MCs) in skin repair is still lacking. Studies in mice examining the role of MC function in the physiology and pathology of skin regenerative processes have obtained contradictory results. To clarify the specific role of MCs in regenerative conditions, here we used a recently developed genetic mouse model that allows conditional MC ablation to examine MC-specific functions in skin. This mouse model is based on the cell type specific expression of Cre reconnbinase in connective tissue type MCs under control of the Mcpt5 promoter and the Cre-inducible diphtheria toxin receptor mediated cell lineage ablation by diphtheria toxin. In response to excisional skin injury, genetic ablation of MCs did not affect the kinetics of reepithelialization, the formation of vascularized granulation tissue, or scar formation. Furthermore, genetic ablation of MCs failed to prevent the development of skin fibrosis upon bleomycin challenge. The amount of deposited collagen and the biochemistry of collagen fibril crosslinks within fibrotic lesions were comparable in MC-depleted and control mice. Collectively, our findings strongly suggest that significant reduction of MC numbers does not affect skin wound healing and bleomycin-induced fibrosis in mice, and provide to our knowledge previously unreported insight in the long-debated contribution of MCs in skin regenerative processes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Willenborg, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Eckes, Beate UNSPECIFIED UNSPECIFIED UNSPECIFIED Brinckmann, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Krieg, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Waisman, Ari UNSPECIFIED orcid.org/0000-0003-4304-8234 UNSPECIFIED Hartmann, Karin UNSPECIFIED orcid.org/0000-0002-4595-8226 UNSPECIFIED Roers, Axel UNSPECIFIED UNSPECIFIED UNSPECIFIED Eming, Sabine A. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-435334
DOI:	10.1038/jid.2014.12
Journal or Publication Title:	J. Invest. Dermatol.
Volume:	134
Number:	7
Page Range:	S. 2005 - 2016
Date:	2014
Publisher:	ELSEVIER SCIENCE INC
Place of Publication:	NEW YORK
ISSN:	1523-1747
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SCLEROTIC SKIN; ANIMAL-MODEL; IN-VIVO; MICE; SCLERODERMA; MECHANISMS; COLLAGEN; INTERLEUKIN-4; REGENERATION; INFLAMMATION Multiple languages Dermatology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43533