Universität zu Köln

Lipp, Melanie, Bucher, Franziska, Parthasarathy, Anand, Hos, Deniz, Onderka, Jasmine, Cursiefen, Claus and Bock, Felix (2014). Blockade of the VEGF isoforms in inflammatory corneal hemangiogenesis and lymphangiogenesis. Graefes Arch. Clin. Exp. Ophthalmol., 252 (6). S. 943 - 950. NEW YORK: SPRINGER. ISSN 1435-702X

Full text not available from this repository.

Abstract

Background The VEGF-A family plays a crucial role in the induction of pathological corneal neovascularization. The role of the different VEGF-A isoforms during lymphangiogenesis is only little-known. Current anti-angiogenic therapies in the eye and other organs inhibit all VEGF-A isoforms, and have effects on both blood and lymphatic vessels. Here we investigate whether selective targeting of the isoform VEGF 165 is able to inhibit corneal lymphangiogenesis under inflammatory conditions. Methods The mouse model of suture-induced corneal neovascularization was used to assess the antihem- and antilymphangiogenic effect of topically applied pegaptanib. Corneal blood and lymph vascularized areas were analyzed morphometrically. Furthermore, we analyzed the proliferative effects of VEGF A 121, 165, and 189 on blood and lymphatic endothelial cells (BEC/LEC) via a cell-proliferation assay. Results Pegaptanib significantly inhibited inflammatory corneal hemangiogenesis (p<0.01), but not lymphangiogenesis in vivo (p>0.05), both topically as well as systemically, in the inflamed cornea. In vitro, BECs were more susceptible to pegaptanib than LECs. Conclusions Targeting VEGF-A 165 significantly inhibits hem-but not lymphangiogenesis, suggesting VEGF-A 165 to be critical for hem-, but dispensable for lymphangiogenesis, at least in the inflamed cornea.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lipp, Melanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Bucher, Franziska UNSPECIFIED UNSPECIFIED UNSPECIFIED Parthasarathy, Anand UNSPECIFIED UNSPECIFIED UNSPECIFIED Hos, Deniz UNSPECIFIED UNSPECIFIED UNSPECIFIED Onderka, Jasmine UNSPECIFIED UNSPECIFIED UNSPECIFIED Cursiefen, Claus UNSPECIFIED UNSPECIFIED UNSPECIFIED Bock, Felix UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-436554
DOI:	10.1007/s00417-014-2626-2
Journal or Publication Title:	Graefes Arch. Clin. Exp. Ophthalmol.
Volume:	252
Number:	6
Page Range:	S. 943 - 950
Date:	2014
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1435-702X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ENDOTHELIAL GROWTH-FACTOR; INTRAVITREAL BEVACIZUMAB; MACULAR DEGENERATION; GRAFT-SURVIVAL; EYE DROPS; ANGIOGENESIS; NEOVASCULARIZATION; INHIBITION; RANIBIZUMAB; PEGAPTANIB Multiple languages Ophthalmology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43655