Universität zu Köln

Matano, Christian, Uhde, Andreas, Youn, Jung-Won, Maeda, Tomoya, Clermont, Lina, Marin, Kay, Kraemer, Reinhard, Wendisch, Volker F. ORCID: 0000-0003-3473-0012 and Seibold, Gerd M. (2014). Engineering of Corynebacterium glutamicum for growth and L-lysine and lycopene production from N-acetyl-glucosamine. Appl. Microbiol. Biotechnol., 98 (12). S. 5633 - 5644. NEW YORK: SPRINGER. ISSN 1432-0614

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Abstract

Sustainable supply of feedstock has become a key issue in process development in microbial biotechnology. The workhorse of industrial amino acid production Corynebacterium glutamicum has been engineered towards utilization of alternative carbon sources. Utilization of the chitin-derived aminosugar N-acetyl-glucosamine (GlcNAc) for both cultivation and production with C. glutamicum has hitherto not been investigated. Albeit this organism harbors the enzymes N-acetylglucosamine-6-phosphatedeacetylase and glucosamine-6P deaminase of GlcNAc metabolism (encoded by nagA and nagB, respectively) growth of C. glutamicum with GlcNAc as substrate was not observed. This was attributed to the lack of a functional system for GlcNAc uptake. Of the 17 type strains of the genus Corynebacterium tested here for their ability to grow with GlcNAc, only Corynebacterium glycinophilum DSM45794 was able to utilize this substrate. Complementation studies with a GlcNAc-uptake deficient Escherichia coli strain revealed that C. glycinophilum possesses a nagE-encoded EII permease for GlcNAc uptake. Heterologous expression of the C. glycinophilum nagE in C. glutamicum indeed enabled uptake of GlcNAc. For efficient GlcNac utilization in C. glutamicum, improved expression of nagE with concurrent overexpression of the endogenous nagA and nagB genes was found to be necessary. Based on this strategy, C. glutamicum strains for the efficient production of the amino acid l-lysine as well as the carotenoid lycopene from GlcNAc as sole substrate were constructed.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Matano, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Uhde, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Youn, Jung-Won UNSPECIFIED UNSPECIFIED UNSPECIFIED Maeda, Tomoya UNSPECIFIED UNSPECIFIED UNSPECIFIED Clermont, Lina UNSPECIFIED UNSPECIFIED UNSPECIFIED Marin, Kay UNSPECIFIED UNSPECIFIED UNSPECIFIED Kraemer, Reinhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Wendisch, Volker F. UNSPECIFIED orcid.org/0000-0003-3473-0012 UNSPECIFIED Seibold, Gerd M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-437317
DOI:	10.1007/s00253-014-5676-9
Journal or Publication Title:	Appl. Microbiol. Biotechnol.
Volume:	98
Number:	12
Page Range:	S. 5633 - 5644
Date:	2014
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-0614
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PHOSPHOTRANSFERASE SYSTEM; ESCHERICHIA-COLI; ALPHA-AMYLASE; ACETYLGLUCOSAMINE UTILIZATION; ETHANOL-PRODUCTION; METABOLIC PATHWAY; ACID PRODUCTION; SOLUBLE STARCH; AMINO-ACIDS; EXPRESSION Multiple languages Biotechnology & Applied Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43731