Universität zu Köln

Marathe, Rahul, Meel, Claudia, Schmidt, Nora C., Dewenter, Lena, Kurre, Rainer, Greune, Lilo, Schmidt, M. Alexander, Mueller, Melanie J. I., Lipowsky, Reinhard ORCID: 0000-0001-8417-8567, Maier, Berenike ORCID: 0000-0001-6971-9927 and Klumpp, Stefan ORCID: 0000-0003-0584-2146 (2014). Bacterial twitching motility is coordinated by a two-dimensional tug-of-war with directional memory. Nat. Commun., 5. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

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Abstract

Type IV pili are ubiquitous bacterial motors that power surface motility. In peritrichously piliated species, it is unclear how multiple pili are coordinated to generate movement with directional persistence. Here we use a combined theoretical and experimental approach to test the hypothesis that multiple pili of Neisseria gonorrhoeae are coordinated through a tug-of-war. Based on force-dependent unbinding rates and pilus retraction speeds measured at the level of single pili, we build a tug-of-war model. Whereas the one-dimensional model robustly predicts persistent movement, the two-dimensional model requires a mechanism of directional memory provided by re-elongation of fully retracted pili and pilus bundling. Experimentally, we confirm memory in the form of bursts of pilus retractions. Bursts are seen even with bundling suppressed, indicating re-elongation from stable core complexes as the key mechanism of directional memory. Directional memory increases the surface range explored by motile bacteria and likely facilitates surface colonization.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Marathe, Rahul UNSPECIFIED UNSPECIFIED UNSPECIFIED Meel, Claudia UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Nora C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Dewenter, Lena UNSPECIFIED UNSPECIFIED UNSPECIFIED Kurre, Rainer UNSPECIFIED UNSPECIFIED UNSPECIFIED Greune, Lilo UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, M. Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Melanie J. I. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lipowsky, Reinhard UNSPECIFIED orcid.org/0000-0001-8417-8567 UNSPECIFIED Maier, Berenike UNSPECIFIED orcid.org/0000-0001-6971-9927 UNSPECIFIED Klumpp, Stefan UNSPECIFIED orcid.org/0000-0003-0584-2146 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-438769
DOI:	10.1038/ncomms4759
Journal or Publication Title:	Nat. Commun.
Volume:	5
Date:	2014
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	2041-1723
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Physics > Institut für Biologische Physik
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IV PILUS SYSTEM; NATURAL TRANSFORMATION; NEISSERIA-GONORRHOEAE; CARGO TRANSPORT; MOTORS; TRANSCRIPTION; COMPETENCE; RETRACTION; COMPONENTS; MECHANISM Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/43876