Universität zu Köln

Esser, Christian, Kuhn, Anke, Groth, Georg, Lercher, Martin J. and Maurino, Veronica G. (2014). Plant and Animal Glycolate Oxidases Have a Common Eukaryotic Ancestor and Convergently Duplicated to Evolve Long-Chain 2-Hydroxy Acid Oxidases. Mol. Biol. Evol., 31 (5). S. 1089 - 1102. OXFORD: OXFORD UNIV PRESS. ISSN 1537-1719

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Abstract

Glycolate oxidase (GOX) is a crucial enzyme of plant photorespiration. The encoding gene is thought to have originated from endosymbiotic gene transfer between the eukaryotic host and the cyanobacterial endosymbiont at the base of plantae. However, animals also possess GOX activities. Plant and animal GOX belong to the gene family of (L)-2-hydroxyacid-oxidases ((L)-2-HAOX). We find that all (L)-2-HAOX proteins in animals and archaeplastida go back to one ancestral eukaryotic sequence; the sole exceptions are green algae of the chlorophyta lineage. Chlorophyta replaced the ancestral eukaryotic (L)-2-HAOX with a bacterial ortholog, a lactate oxidase that may have been obtained through the primary endosymbiosis at the base of plantae; independent losses of this gene may explain its absence in other algal lineages (glaucophyta, rhodophyta, and charophyta). We also show that in addition to GOX, plants possess (L)-2-HAOX proteins with different specificities for medium- and long-chain hydroxyacids (lHAOX), likely involved in fatty acid and protein catabolism. Vertebrates possess lHAOX proteins acting on similar substrates as plant lHAOX; however, the existence of GOX and lHAOX subfamilies in both plants and animals is not due to shared ancestry but is the result of convergent evolution in the two most complex eukaryotic lineages. On the basis of targeting sequences and predicted substrate specificities, we conclude that the biological role of plantae (L)-2-HAOX in photorespiration evolved by co-opting an existing peroxisomal protein.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Esser, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuhn, Anke UNSPECIFIED UNSPECIFIED UNSPECIFIED Groth, Georg UNSPECIFIED UNSPECIFIED UNSPECIFIED Lercher, Martin J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Maurino, Veronica G. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-439174
DOI:	10.1093/molbev/msu041
Journal or Publication Title:	Mol. Biol. Evol.
Volume:	31
Number:	5
Page Range:	S. 1089 - 1102
Date:	2014
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1537-1719
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language L-LACTATE UTILIZATION; PHOTORESPIRATORY GLYCOLATE; SUBSTRATE-SPECIFICITY; AEROCOCCUS-VIRIDANS; PROTEIN EVOLUTION; HUMAN FIBROBLASTS; ESCHERICHIA-COLI; PHYTANIC ACID; GENE-TRANSFER; MIXED MODELS Multiple languages Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43917