Universität zu Köln

Preethi, Jayakumar, Singh, Hemant K., Venkataraman, Jois Shreyas and Rajan, Koilmani Emmanuvel (2014). Standardised Extract of Bacopa monniera (CDRI-08) Improves Contextual Fear Memory by Differentially Regulating the Activity of Histone Acetylation and Protein Phosphatases (PP1 alpha, PP2A) in Hippocampus. Cell. Mol. Neurobiol., 34 (4). S. 577 - 590. NEW YORK: SPRINGER/PLENUM PUBLISHERS. ISSN 1573-6830

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Abstract

Contextual fear conditioning is a paradigm for investigating cellular mechanisms involved in hippocampus-dependent memory. Earlier, we showed that standardised extract of Bacopa monniera (CDRI-08) improves hippocampus-dependent learning in postnatal rats by elevating the level of serotonin (5-hydroxytryptamine, 5-HT), activate 5-HT3A receptors, and cyclic adenosine monophosphate (cAMP) response element binding (CREB) protein. In this study, we have further examined the molecular mechanism of CDRI-08 in hippocampus-dependent memory and compared to the histone deacetylase (HDACs) inhibitor sodium butyrate (NaB). To assess the hippocampus-dependent memory, wistar rat pups were subjected to contextual fear conditioning (CFC) following daily (postnatal days 15-29) administration of vehicle solution (0.5 % gum acacia + 0.9 % saline)/CDRI-08 (80 mg/kg, p.o.)/NaB (1.2 g/kg in PBS, i.p.). CDRI-08/NaB treated group showed enhanced freezing behavior compared to control group when re-exposed to the same context. Administration of CDRI-08/NaB resulted in activation of extracellular signal-regulated kinase ERK/CREB signaling cascade and up-regulation of p300, Ac-H3 and Ac-H4 levels, and down-regulation of HDACs (1, 2) and protein phosphatases (PP1 alpha, PP2A) in hippocampus following CFC. This would subsequently result in an increased brain-derived neurotrophic factor (Bdnf) (exon IV) mRNA in hippocampus. Altogether, our results indicate that CDRI-08 enhances hippocampus-dependent contextual memory by differentially regulating histone acetylation and protein phosphatases in hippocampus.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Preethi, Jayakumar UNSPECIFIED UNSPECIFIED UNSPECIFIED Singh, Hemant K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Venkataraman, Jois Shreyas UNSPECIFIED UNSPECIFIED UNSPECIFIED Rajan, Koilmani Emmanuvel UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-439529
DOI:	10.1007/s10571-014-0042-0
Journal or Publication Title:	Cell. Mol. Neurobiol.
Volume:	34
Number:	4
Page Range:	S. 577 - 590
Date:	2014
Publisher:	SPRINGER/PLENUM PUBLISHERS
Place of Publication:	NEW YORK
ISSN:	1573-6830
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BDNF GENE-TRANSCRIPTION; SYNAPTIC PLASTICITY; ACETYLTRANSFERASE ACTIVITY; CHEMICAL EXAMINATION; DORSAL HIPPOCAMPUS; COACTIVATORS P300; BINDING PROTEIN; CREB; EXPRESSION; BRAIN Multiple languages Cell Biology; Neurosciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43952