Universität zu Köln

Muhl, Daniela, Kathmann, Markus, Hoyer, Carolin, Kranaster, Laura, Hellmich, Martin, Gerth, Christoph W., Faulhaber, Johannes, Schlicker, Eberhard and Leweke, F. Markus ORCID: 0000-0002-8163-195X (2014). Increased CB2 mRNA and anandamide in human blood after cessation of cannabis abuse. Naunyn-Schmiedebergs Arch. Pharmacol., 96.6151338822223 (5.03680674372991). S. 1276.88747346072 - 1283.6519904465. NEW YORK: SPRINGER. ISSN 1432-1913

Full text not available from this repository.

Abstract

In previous studies, long-term cannabis use led to alterations of the endocannabinoid system including an increase in CB1 and/or CB2 receptor messenger RNA (mRNA) in blood cells and an increase in the serum level of the endocannabinoid 2-arachidonoyl glycerol. However, in those studies, cannabis use was stopped only few days before testing or not interrupted at all. Therefore, one cannot decide whether the alterations are due to long-term cannabis abuse or are confounded by acute effects of cannabis. Blood was sampled from donors that had smoked marijuana a parts per thousand yen20 times in their lives but had abstained from cannabis for a parts per thousand yen6 months (high-frequency users, HFU) and from controls (cannabis use a parts per thousand currency sign5 times lifetime). CB1 and CB2 mRNA was determined in peripheral mononuclear blood cells using the reverse transcriptase polymerase chain reaction. Serum anandamide level was assayed using electrospray tandem mass spectrometry. CB2 mRNA was increased by 45 % in HFU when compared to controls, whereas CB1 mRNA did not differ. The anandamide level in HFU exceeded that in controls by 90 %. Tobacco smoking could be excluded as a confounding factor. In conclusion, marijuana users that had smoked marijuana a parts per thousand yen20 times in their lives and stopped cannabis use at least 6 months before the study show an increase in CB2 receptor mRNA in the blood and in serum anandamide level. These alterations resemble those obtained for marijuana smokers that had stopped cannabis use only few days before testing and may be implicated in the pathogenesis of disorders associated with long-term cannabis use.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Muhl, Daniela UNSPECIFIED UNSPECIFIED UNSPECIFIED Kathmann, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoyer, Carolin UNSPECIFIED UNSPECIFIED UNSPECIFIED Kranaster, Laura UNSPECIFIED UNSPECIFIED UNSPECIFIED Hellmich, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Gerth, Christoph W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Faulhaber, Johannes UNSPECIFIED UNSPECIFIED UNSPECIFIED Schlicker, Eberhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Leweke, F. Markus UNSPECIFIED orcid.org/0000-0002-8163-195X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-439684
DOI:	10.1007/s00210-014-0984-3
Journal or Publication Title:	Naunyn-Schmiedebergs Arch. Pharmacol.
Volume:	96.6151338822223
Number:	5.03680674372991
Page Range:	S. 1276.88747346072 - 1283.6519904465
Date:	2014
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-1913
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CEREBROSPINAL-FLUID; PSYCHOTIC SYMPTOMS; SCHIZOPHRENIA; EXPRESSION; RECEPTORS; SYSTEM; BRAIN Multiple languages Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/43968