Universität zu Köln

Gangel, Heike, Hepp, Christof, Mueller, Stephanie, Oldewurtel, Enno R., Aas, Finn Erik, Koomey, Michael ORCID: 0000-0001-5844-2344 and Maier, Berenike ORCID: 0000-0001-6971-9927 (2014). Concerted Spatio-Temporal Dynamics of Imported DNA and ComE DNA Uptake Protein during Gonococcal Transformation. PLoS Pathog., 10 (4). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1553-7374

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Abstract

Competence for transformation is widespread among bacterial species. In the case of Gram-negative systems, a key step to transformation is the import of DNA across the outer membrane. Although multiple factors are known to affect DNA transport, little is known about the dynamics of DNA import. Here, we characterized the spatio-temporal dynamics of DNA import into the periplasm of Neisseria gonorrhoeae. DNA was imported into the periplasm at random locations around the cell contour. Subsequently, it was recruited at the septum of diplococci at a time scale that increased with DNA length. We found using fluorescent DNA that the periplasm was saturable within minutes with similar to 40 kbp DNA. The DNA-binding protein ComE quantitatively governed the carrying capacity of the periplasm in a gene-dosage-dependent fashion. As seen using a fluorescent-tagged derivative protein, ComE was homogeneously distributed in the periplasm in the absence of external DNA. Upon addition of external DNA, ComE was relocalized to form discrete foci colocalized with imported DNA. We conclude that the periplasm can act as a considerable reservoir for imported DNA with ComE governing the amount of DNA stored potentially for transport through the inner membrane.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gangel, Heike UNSPECIFIED UNSPECIFIED UNSPECIFIED Hepp, Christof UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Stephanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Oldewurtel, Enno R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Aas, Finn Erik UNSPECIFIED UNSPECIFIED UNSPECIFIED Koomey, Michael UNSPECIFIED orcid.org/0000-0001-5844-2344 UNSPECIFIED Maier, Berenike UNSPECIFIED orcid.org/0000-0001-6971-9927 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-440927
DOI:	10.1371/journal.ppat.1004043
Journal or Publication Title:	PLoS Pathog.
Volume:	10
Number:	4
Date:	2014
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1553-7374
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Physics > Institut für Biologische Physik
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IV PILUS EXPRESSION; COMPETENT BACILLUS-SUBTILIS; NEISSERIA-GONORRHOEAE; NATURAL TRANSFORMATION; VIBRIO-CHOLERAE; PILIN; BINDING; COMPONENTS; IDENTIFICATION; TRANSLOCATION Multiple languages Microbiology; Parasitology; Virology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/44092