Universität zu Köln

Krajinski, Franziska, Courty, Pierre-Emmanuel, Sieh, Daniela, Franken, Philipp, Zhang, Haoqiang, Bucher, Marcel ORCID: 0000-0003-1680-9413, Gerlach, Nina, Kryvoruchko, Igor, Zoeller, Daniela, Udvardi, Michael ORCID: 0000-0001-9850-0828 and Hause, Bettina (2014). The H+-ATPase HA1 of Medicago truncatula Is Essential for Phosphate Transport and Plant Growth during Arbuscular Mycorrhizal Symbiosis. Plant Cell, 26 (4). S. 1808 - 1818. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1532-298X

Full text not available from this repository.

Abstract

A key feature of arbuscular mycorrhizal symbiosis is improved phosphorus nutrition of the host plant via the mycorrhizal pathway, i.e., the fungal uptake of Pi from the soil and its release from arbuscules within root cells. Efficient transport of Pi from the fungus to plant cells is thought to require a proton gradient across the periarbuscular membrane (PAM) that separates fungal arbuscules from the host cell cytoplasm. Previous studies showed that the H+-ATPase gene HA1 is expressed specifically in arbuscule containing root cells of Medicago truncatula. We isolated a ha1-2 mutant of M. truncatula and found it to be impaired in the development of arbuscules but not in root colonization by Rhizophagus irregularis hyphae. Artificial microRNA silencing of HA1 recapitulated this phenotype, resulting in small and truncated arbuscules. Unlike the wild type, the ha1-2 mutant failed to show a positive growth response to mycorrhizal colonization under Pi-limiting conditions. Uptake experiments confirmed that ha1-2 mutants are unable to take up phosphate via the mycorrhizal pathway. Increased pH in the apoplast of abnormal arbuscule containing cells of the ha1-2 mutant compared with the wild type suggests that HA1 is crucial for building a proton gradient across the PAM and therefore is indispensible for the transfer of Pi from the fungus to the plant.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Krajinski, Franziska UNSPECIFIED UNSPECIFIED UNSPECIFIED Courty, Pierre-Emmanuel UNSPECIFIED UNSPECIFIED UNSPECIFIED Sieh, Daniela UNSPECIFIED UNSPECIFIED UNSPECIFIED Franken, Philipp UNSPECIFIED UNSPECIFIED UNSPECIFIED Zhang, Haoqiang UNSPECIFIED UNSPECIFIED UNSPECIFIED Bucher, Marcel UNSPECIFIED orcid.org/0000-0003-1680-9413 UNSPECIFIED Gerlach, Nina UNSPECIFIED UNSPECIFIED UNSPECIFIED Kryvoruchko, Igor UNSPECIFIED UNSPECIFIED UNSPECIFIED Zoeller, Daniela UNSPECIFIED UNSPECIFIED UNSPECIFIED Udvardi, Michael UNSPECIFIED orcid.org/0000-0001-9850-0828 UNSPECIFIED Hause, Bettina UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-440993
DOI:	10.1105/tpc.113.120436
Journal or Publication Title:	Plant Cell
Volume:	26
Number:	4
Page Range:	S. 1808 - 1818
Date:	2014
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	1532-298X
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RHIZOGENES-TRANSFORMED ROOTS; NUTRIENT-UPTAKE; FUNGI; GENE; EXPRESSION; NITROGEN; HOST; TRANSCRIPTION; NUTRITION; PROTEIN Multiple languages Biochemistry & Molecular Biology; Plant Sciences; Cell Biology Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/44099