Aksoy, Irene ORCID: 0000-0003-4353-5876, Giudice, Vincent, Delahaye, Edwige, Wianny, Florence, Aubry, Maxime, Mure, Magali, Chen, Jiaxuan, Jauch, Ralf ORCID: 0000-0002-6590-9579, Bogu, Gireesh K., Nolden, Tobias, Himmelbauer, Heinz ORCID: 0000-0001-8897-2928, Xavier Doss, Michael, Sachinidis, Agapios, Schulz, Herbert, Hummel, Oliver, Martinelli, Paola ORCID: 0000-0002-1643-8731, Huebner, Norbert, Stanton, Lawrence W., Real, Francisco X., Bourillot, Pierre-Yves ORCID: 0000-0003-1657-8555 and Savatier, Pierre (2014). Klf4 and Klf5 differentially inhibit mesoderm and endoderm differentiation in embryonic stem cells. Nat. Commun., 5. LONDON: NATURE PUBLISHING GROUP. ISSN 2041-1723

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Abstract

Kruppel-like factors (Klf) 4 and 5 are two closely related members of the Klf family, known to play key roles in cell cycle regulation, somatic cell reprogramming and pluripotency. Here we focus on the functional divergence between Klf4 and Klf5 in the inhibition of mouse embryonic stem (ES) cell differentiation. Using microarrays and chromatin immunoprecipitation coupled to ultra-high-throughput DNA sequencing, we show that Klf4 negatively regulates the expression of endodermal markers in the undifferentiated ES cells, including transcription factors involved in the commitment of pluripotent stem cells to endoderm differentiation. Knockdown of Klf4 enhances differentiation towards visceral and definitive endoderm. In contrast, Klf5 negatively regulates the expression of mesodermal markers, some of which control commitment to the mesoderm lineage, and knockdown of Klf5 specifically enhances differentiation towards mesoderm. We conclude that Klf4 and Klf5 differentially inhibit mesoderm and endoderm differentiation in murine ES cells.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Aksoy, IreneUNSPECIFIEDorcid.org/0000-0003-4353-5876UNSPECIFIED
Giudice, VincentUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Delahaye, EdwigeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wianny, FlorenceUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Aubry, MaximeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mure, MagaliUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Chen, JiaxuanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jauch, RalfUNSPECIFIEDorcid.org/0000-0002-6590-9579UNSPECIFIED
Bogu, Gireesh K.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nolden, TobiasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Himmelbauer, HeinzUNSPECIFIEDorcid.org/0000-0001-8897-2928UNSPECIFIED
Xavier Doss, MichaelUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sachinidis, AgapiosUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schulz, HerbertUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hummel, OliverUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Martinelli, PaolaUNSPECIFIEDorcid.org/0000-0002-1643-8731UNSPECIFIED
Huebner, NorbertUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Stanton, Lawrence W.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Real, Francisco X.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bourillot, Pierre-YvesUNSPECIFIEDorcid.org/0000-0003-1657-8555UNSPECIFIED
Savatier, PierreUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-441357
DOI: 10.1038/ncomms4719
Journal or Publication Title: Nat. Commun.
Volume: 5
Date: 2014
Publisher: NATURE PUBLISHING GROUP
Place of Publication: LONDON
ISSN: 2041-1723
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
KRUPPEL-LIKE FACTOR-4; TRANSCRIPTION FACTORS; SELF-RENEWAL; DEFINITIVE ENDODERM; EXPRESSION; GENE; SOX17; ENHANCER; KRUPPEL-LIKE-FACTOR-4; PLURIPOTENCYMultiple languages
Multidisciplinary SciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/44135

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