Universität zu Köln

Eckl, Katja-Martina, Weindl, Guenther, Ackermann, Katharina, Kuechler, Sarah, Casper, Ramona, Radowski, Michal R., Haag, Rainer, Hennies, Hans Christian and Schaefer-Korting, Monika (2014). Increased cutaneous absorption reflects impaired barrier function of reconstructed skin models mimicking keratinisation disorders. Exp. Dermatol., 23 (4). S. 286 - 289. HOBOKEN: WILEY-BLACKWELL. ISSN 1600-0625

Full text not available from this repository.

Abstract

The aim of this study was to assess a recently established 3D model of congenital ichthyosis, representing severe epidermal barrier function defects, for skin penetration and permeation. We have generated disease models by knock-down of either TGM1 or ALOXE3 in primary human keratinocytes, and using keratinocytes and fibroblasts from patients with congenital ichthyosis. The results indicate disturbed barrier function as demonstrated by increased permeation of testosterone and caffeine particularly in TGM1 knock-down models compared to control models. In addition, enhanced penetration of the model dye nile red incorporated into solid lipid nanoparticles and core-multishell nanotransporters, respectively, was evident in disease models. Thus, in vitro skin disease models reproduce differences in barrier permeability and function seen in congenital ichthyosis and pave the way to personalised disease models. Furthermore, our findings indicate that nanocarriers may be useful in new, topical therapeutic approaches for the currently very limited treatment of congenital ichthyosis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Eckl, Katja-Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Weindl, Guenther UNSPECIFIED UNSPECIFIED UNSPECIFIED Ackermann, Katharina UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuechler, Sarah UNSPECIFIED UNSPECIFIED UNSPECIFIED Casper, Ramona UNSPECIFIED UNSPECIFIED UNSPECIFIED Radowski, Michal R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Haag, Rainer UNSPECIFIED UNSPECIFIED UNSPECIFIED Hennies, Hans Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaefer-Korting, Monika UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-441690
DOI:	10.1111/exd.12366
Journal or Publication Title:	Exp. Dermatol.
Volume:	23
Number:	4
Page Range:	S. 286 - 289
Date:	2014
Publisher:	WILEY-BLACKWELL
Place of Publication:	HOBOKEN
ISSN:	1600-0625
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FULL-THICKNESS SKIN; PENETRATION ENHANCEMENT; LIPID NANOPARTICLES; EPIDERMIS Multiple languages Dermatology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44169