Universität zu Köln

Marko, Marija, Leichter, Michael, Patrinou-Georgoula, Meropi and Guialis, Apostolia (2014). Selective interactions of hnRNP M isoforms with the TET proteins TAF15 and TLS/FUS. Mol. Biol. Rep., 41 (4). S. 2687 - 2696. DORDRECHT: SPRINGER. ISSN 1573-4978

Full text not available from this repository.

Abstract

The molecular composition of macromolecular assemblies engaged in transcription and splicing influences biogenesis of mRNA transcripts. Preference for one over the other interactive protein partner within those complexes is expected to change the gene expression pattern and to affect subsequent cellular events. We report here the novel and selective associations between RNA-binding proteins, namely the hnRNP M1-4 isoforms-involved in early spliceosome assembly and alternative splicing-and the transcription factors TAF15 and TLS/FUS. In immunoprecipitation studies on HeLa nuclear extracts, TAF15 co-immunoprecipitates preferably with the higher molecular weight hnRNP M3/4 isoforms, opposite to TLS/FUS that associates with the lower molecular weight hnRNP M1/2 species. We demonstrate that these associations can be mediated through direct protein-protein interactions via the amino-termini of the TET proteins, independently of RNA. Finally, we show partial co-localization of TAF15 and TLS/FUS with hnRNP M proteins in HeLa nuclei, supporting the biochemically obtained data. The participation of hnRNP M in an expanding network of protein-protein interactions suggests its important functioning in the coordination of transcriptional and post-transcriptional events.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Marko, Marija UNSPECIFIED UNSPECIFIED UNSPECIFIED Leichter, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Patrinou-Georgoula, Meropi UNSPECIFIED UNSPECIFIED UNSPECIFIED Guialis, Apostolia UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-441734
DOI:	10.1007/s11033-014-3128-3
Journal or Publication Title:	Mol. Biol. Rep.
Volume:	41
Number:	4
Page Range:	S. 2687 - 2696
Date:	2014
Publisher:	SPRINGER
Place of Publication:	DORDRECHT
ISSN:	1573-4978
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RNA-POLYMERASE-II; NUCLEAR RIBONUCLEOPROTEIN-M; PRE-MESSENGER-RNA; BINDING PROTEIN; FAMILY; EWS; TRANSCRIPTION; TRANSACTIVATION; IDENTIFICATION; LOCALIZATION Multiple languages Biochemistry & Molecular Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44173