Universität zu Köln

Zwolanek, Daniela, Veit, Guido ORCID: 0000-0002-6758-2696, Eble, Johannes A., Gullberg, Donald ORCID: 0000-0002-7245-4437, Ruggiero, Florence, Heino, Jyrki, Meier, Markus ORCID: 0000-0003-1068-746X, Stetefeld, Joerg ORCID: 0000-0003-1478-3248 and Koch, Manuel ORCID: 0000-0002-2962-7814 (2014). Collagen XXII binds to collagen-binding integrins via the novel motifs GLQGER and GFKGER. Biochem. J., 459. S. 217 - 228. LONDON: PORTLAND PRESS LTD. ISSN 1470-8728

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Abstract

Collagen XXII, a FACIT (fibril-associated collagen with interrupted triple helices), is expressed at the myotendinous junction and the articular surface of joint cartilage. Cellular receptors like collagen-binding integrins are known to bind collagens with distinct binding motifs following the sequence GXOGER. In the present study, we demonstrate the sequences GLQGER and GFKGER as novel binding motifs between collagen XXII and collagen-binding integrins, especially alpha 2 beta 1 integrin. Solid-phase assays and surface plasmon resonance spectroscopy revealed a direct interaction between alpha 2 beta 1 integrin and the motif GFKGER. In addition, immunohistochemical analysis demonstrated partial co-localization of collagen XXII, alpha 2 beta 1 integrin and alpha 11 beta 1 integrin at the myotendinous junction. Furthermore, computational modelling of the motifs GLQGER and GFKGER showed perfect fitting of the sequences into the binding pocket of collagen-binding integrins. Taken together, we demonstrated that collagen XXII interacts with collagen-binding integrins via the new motifs GLQGER and GFKGER.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zwolanek, Daniela UNSPECIFIED UNSPECIFIED UNSPECIFIED Veit, Guido UNSPECIFIED orcid.org/0000-0002-6758-2696 UNSPECIFIED Eble, Johannes A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gullberg, Donald UNSPECIFIED orcid.org/0000-0002-7245-4437 UNSPECIFIED Ruggiero, Florence UNSPECIFIED UNSPECIFIED UNSPECIFIED Heino, Jyrki UNSPECIFIED UNSPECIFIED UNSPECIFIED Meier, Markus UNSPECIFIED orcid.org/0000-0003-1068-746X UNSPECIFIED Stetefeld, Joerg UNSPECIFIED orcid.org/0000-0003-1478-3248 UNSPECIFIED Koch, Manuel UNSPECIFIED orcid.org/0000-0002-2962-7814 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-442002
DOI:	10.1042/BJ20130642
Journal or Publication Title:	Biochem. J.
Volume:	459
Page Range:	S. 217 - 228
Date:	2014
Publisher:	PORTLAND PRESS LTD
Place of Publication:	LONDON
ISSN:	1470-8728
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CELL-LINE HACAT; SWISS-MODEL; ALPHA-11-BETA-1 INTEGRIN; ALPHA(2)BETA(1) INTEGRIN; ALPHA-2-BETA-1 INTEGRIN; CRYSTAL-STRUCTURE; PROTEIN; ADHESION; EXPRESSION; ALPHA(1)BETA(1) Multiple languages Biochemistry & Molecular Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44200