Universität zu Köln

Schneider, Anja ORCID: 0000-0002-3377-5828, Steinberger, Iris, Strissel, Henning, Kunz, Hans-Henning, Manavski, Nikolay ORCID: 0000-0003-2740-5991, Meurer, Joerg, Burkhard, Gabi, Jarzombski, Sabine, Schuenemann, Danja ORCID: 0000-0001-8894-7685, Geimer, Stefan, Fluegge, Ulf-Ingo and Leister, Dario ORCID: 0000-0003-1897-8421 (2014). The Arabidopsis Tellurite resistance C protein together with ALB3 is involved in photosystemII protein synthesis. Plant J., 78 (2). S. 344 - 357. HOBOKEN: WILEY-BLACKWELL. ISSN 1365-313X

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Abstract

Assembly of photosystemII (PSII) occurs sequentially and requires several auxiliary proteins, such as ALB3 (ALBINO3). Here, we describe the role of the Arabidopsis thaliana thylakoid membrane protein Tellurite resistance C (AtTerC) in this process. Knockout of AtTerC was previously shown to be seedling-lethal. This phenotype was rescued by expressing TerC fused C-terminally to GFP in the terc-1 background, and the resulting terc-1(TerC-)(GFP) line and an artificial miRNA-based knockdown allele (amiR-TerC) were used to analyze the TerC function. The alterations in chlorophyll fluorescence and thylakoid ultrastructure observed in amiR-TerC plants and terc-1(TerC-)(GFP) were attributed to defects in PSII. We show that this phenotype resulted from a reduction in the rate of de novo synthesis of PSII core proteins, but later steps in PSII biogenesis appeared to be less affected. Yeast two-hybrid assays showed that TerC interacts with PSII proteins. In particular, its interaction with the PSII assembly factor ALB3 has been demonstrated by co-immunoprecipitation. ALB3 is thought to assist in incorporation of CP43 into PSII via interaction with Low PSII Accumulation2 (LPA2) Low PSII Accumulation3 (LPA3). Homozygous lpa2 mutants expressing amiR-TerC displayed markedly exacerbated phenotypes, leading to seedling lethality, indicating an additive effect. We propose a model in which TerC, together with ALB3, facilitates de novo synthesis of thylakoid membrane proteins, for instance CP43, at the membrane insertion step.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schneider, Anja UNSPECIFIED orcid.org/0000-0002-3377-5828 UNSPECIFIED Steinberger, Iris UNSPECIFIED UNSPECIFIED UNSPECIFIED Strissel, Henning UNSPECIFIED UNSPECIFIED UNSPECIFIED Kunz, Hans-Henning UNSPECIFIED UNSPECIFIED UNSPECIFIED Manavski, Nikolay UNSPECIFIED orcid.org/0000-0003-2740-5991 UNSPECIFIED Meurer, Joerg UNSPECIFIED UNSPECIFIED UNSPECIFIED Burkhard, Gabi UNSPECIFIED UNSPECIFIED UNSPECIFIED Jarzombski, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED Schuenemann, Danja UNSPECIFIED orcid.org/0000-0001-8894-7685 UNSPECIFIED Geimer, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Fluegge, Ulf-Ingo UNSPECIFIED UNSPECIFIED UNSPECIFIED Leister, Dario UNSPECIFIED orcid.org/0000-0003-1897-8421 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-442842
DOI:	10.1111/tpj.12474
Journal or Publication Title:	Plant J.
Volume:	78
Number:	2
Page Range:	S. 344 - 357
Date:	2014
Publisher:	WILEY-BLACKWELL
Place of Publication:	HOBOKEN
ISSN:	1365-313X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language OXYGEN-EVOLVING COMPLEX; II REACTION-CENTER; THYLAKOID MEMBRANE; PLANT TRANSFORMATION; EXTRINSIC PROTEINS; BINDING PROTEIN; GENE-EXPRESSION; D1 PROTEIN; IN-VIVO; CHLOROPLAST Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44284