Universität zu Köln

Rubach, Martin, Adelmann, Roland, Haustein, Moritz, Drey, Florian, Pfannkuche, Kurt, Xiao, Bing, Koester, Annette, ten Cate, Floris E. A. Udink, Choi, Yeong-Hoon, Neef, Klaus, Fatima, Azra, Hannes, Tobias ORCID: 0000-0002-8550-2282, Pillekamp, Frank, Hescheler, Juergen, Saric, Tomo ORCID: 0000-0001-8344-1095, Brockmeier, Konrad and Khalil, Markus (2014). Mesenchymal Stem Cells and Their Conditioned Medium Improve Integration of Purified Induced Pluripotent Stem Cell-Derived Cardiomyocyte Clusters into Myocardial Tissue. Stem Cells Dev., 23 (6). S. 643 - 654. NEW ROCHELLE: MARY ANN LIEBERT, INC. ISSN 1557-8534

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Abstract

Induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) might become therapeutically relevant to regenerate myocardial damage. Purified iPS-CMs exhibit poor functional integration into myocardial tissue. The aim of this study was to investigate whether murine mesenchymal stem cells (MSCs) or their conditioned medium (MScond) improves the integration of murine iPS-CMs into myocardial tissue. Vital or nonvital embryonic murine ventricular tissue slices were cocultured with purified clusters of iPS-CMs in combination with murine embryonic fibroblasts (MEFs), MSCs, or MScond. Morphological integration was assessed by visual scoring and functional integration by isometric force and field potential measurements. We observed a moderate morphological integration of iPS-CM clusters into vital, but a poor integration into nonvital, slices. MEFs and MSCs but not MScond improved morphological integration of CMs into nonvital slices and enabled purified iPS-CMs to confer force. Coculture of vital slices with iPS-CMs and MEFs or MSCs resulted in an improved electrical integration. A comparable improvement of electrical coupling was achieved with the cell-free MScond, indicating that soluble factors secreted by MSCs were involved in electrical coupling. We conclude that cells such as MSCs support the engraftment and adhesion of CMs, and confer force to noncontractile tissue. Furthermore, soluble factors secreted by MSCs mediate electrical coupling of purified iPS-CM clusters to myocardial tissue. These data suggest that MSCs may increase the functional engraftment and therapeutic efficacy of transplanted iPS-CMs into infarcted myocardium.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Rubach, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Adelmann, Roland UNSPECIFIED UNSPECIFIED UNSPECIFIED Haustein, Moritz UNSPECIFIED UNSPECIFIED UNSPECIFIED Drey, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED Pfannkuche, Kurt UNSPECIFIED UNSPECIFIED UNSPECIFIED Xiao, Bing UNSPECIFIED UNSPECIFIED UNSPECIFIED Koester, Annette UNSPECIFIED UNSPECIFIED UNSPECIFIED ten Cate, Floris E. A. Udink UNSPECIFIED UNSPECIFIED UNSPECIFIED Choi, Yeong-Hoon UNSPECIFIED UNSPECIFIED UNSPECIFIED Neef, Klaus UNSPECIFIED UNSPECIFIED UNSPECIFIED Fatima, Azra UNSPECIFIED UNSPECIFIED UNSPECIFIED Hannes, Tobias UNSPECIFIED orcid.org/0000-0002-8550-2282 UNSPECIFIED Pillekamp, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Saric, Tomo UNSPECIFIED orcid.org/0000-0001-8344-1095 UNSPECIFIED Brockmeier, Konrad UNSPECIFIED UNSPECIFIED UNSPECIFIED Khalil, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-443366
DOI:	10.1089/scd.2013.0272
Journal or Publication Title:	Stem Cells Dev.
Volume:	23
Number:	6
Page Range:	S. 643 - 654
Date:	2014
Publisher:	MARY ANN LIEBERT, INC
Place of Publication:	NEW ROCHELLE
ISSN:	1557-8534
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IN-VITRO TRANSPLANTATION; INFARCTION; FIBROBLASTS; ADULT; REGENERATION; EXPRESSION; HEART; DIFFERENTIATION; RECRUITMENT; ENGRAFTMENT Multiple languages Cell & Tissue Engineering; Hematology; Medicine, Research & Experimental; Transplantation Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44336