Universität zu Köln

Hardt, Anna-Lena, Schildgen, Verena, Tillmann, Ramona-Liza, Brockmann, Michael and Schildgen, Oliver ORCID: 0000-0003-4297-9627 (2014). Utility of the QIAGEN/artus PCR assays and Meningofinder (TM) for the detection of human herpes virus genomes in formalin-fixed paraffin-embedded clinical samples. Future Virol., 9 (3). S. 241 - 243. LONDON: FUTURE MEDICINE LTD. ISSN 1746-0808

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Abstract

Aim: The aim of the study was to analyze whether human herpes viruses can be detected from formalin-fixed paraffin-embedded (FFPE) tissues with two commercial methods. Materials & methods: In total, 91 clinical FFPE samples were analyzed with QIAGEN/ artus (Hilden, Germany) qPCRs and the Meningofinder (TM) assay from PathoFinder (Maastricht, The Netherlands). Results: A total of 27 out of 91 samples were tested for HSV-1 and HSV-2. Three of these 27 samples (11.1%) were positive for HSV-1 and one sample was positive for HSV-2. Out of 91 samples, 50 were tested for CMV, of which six samples were positive (12%). In total, 12 out of 91 samples were tested for the presence of Varizella zoster virus DNA, revealing one as positive (8%). Finally, two samples were tested negative for EBV. Conclusion: Minor differences were identified between both assays, making them a reliable tool for detection of herpes viruses from FFPE material, although this is an off-label use for both assays.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hardt, Anna-Lena UNSPECIFIED UNSPECIFIED UNSPECIFIED Schildgen, Verena UNSPECIFIED UNSPECIFIED UNSPECIFIED Tillmann, Ramona-Liza UNSPECIFIED UNSPECIFIED UNSPECIFIED Brockmann, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Schildgen, Oliver UNSPECIFIED orcid.org/0000-0003-4297-9627 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-443984
DOI:	10.2217/FVL.14.7
Journal or Publication Title:	Future Virol.
Volume:	9
Number:	3
Page Range:	S. 241 - 243
Date:	2014
Publisher:	FUTURE MEDICINE LTD
Place of Publication:	LONDON
ISSN:	1746-0808
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TISSUE Multiple languages Virology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44398