Universität zu Köln

Voelkl, Jakob, Alesutan, Ioana, Pakladok, Tatsiana, Viereck, Robert, Feger, Martina, Mia, Sobuj, Schoenberger, Tanja, Noegel, Angelika A., Gawaz, Meinrad and Lang, Florian (2014). Annexin A7 deficiency potentiates cardiac NFAT activity promoting hypertrophic signaling. Biochem. Biophys. Res. Commun., 445 (1). S. 244 - 250. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 1090-2104

Full text not available from this repository.

Abstract

Annexin A7 (Anxa7) is a cytoskeletal protein interacting with Ca2+ signaling which in turn is a crucial factor for cardiac remodeling following cardiac injury. The present study explored whether Anxa7 participates in the regulation of cardiac stress signaling. To this end, mice lacking functional Anxa7 (anxa7(-/-)) and wild-type mice (anxa7(+/+)) were investigated following pressure overload by transverse aortic constriction (TAC). In addition, HL-1 cardiomyocytes were silenced with Anxa7 siRNA and treated with isoproterenol. Transcript levels were determined by quantitative RT-PCR, transcriptional activity by luciferase reporter assay and protein abundance by Western blotting and confocal microscopy. As a result, TAC treatment increased the mRNA and protein levels of Anxa7 in wild-type mice. Moreover, TAC increased heart weight to body weight ratio and the cardiac mRNA levels of alpha Ska, Nppb, Col1a1, Col3a1 and Rcan1, effects more pronounced in anxa7(-/-) mice than in anxa7(+/+) mice. Silencing of Anxa7 in HL-1 cardiomyocytes significantly increased nuclear localization of Nfatc1. Furthermore, Anxa7 silencing increased NFAT-dependent transcriptional activity as well as alpha Ska, Nppb, and Rcan1 mRNA levels both, under control conditions and following beta-adrenergic stimulation by isoproterenol. These observations point to an important role of annexin A7 in the regulation of cardiac NFAT activity and hypertrophic response following cardiac stress conditions. (C) 2014 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Voelkl, Jakob UNSPECIFIED UNSPECIFIED UNSPECIFIED Alesutan, Ioana UNSPECIFIED UNSPECIFIED UNSPECIFIED Pakladok, Tatsiana UNSPECIFIED UNSPECIFIED UNSPECIFIED Viereck, Robert UNSPECIFIED UNSPECIFIED UNSPECIFIED Feger, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Mia, Sobuj UNSPECIFIED UNSPECIFIED UNSPECIFIED Schoenberger, Tanja UNSPECIFIED UNSPECIFIED UNSPECIFIED Noegel, Angelika A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gawaz, Meinrad UNSPECIFIED UNSPECIFIED UNSPECIFIED Lang, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-445632
DOI:	10.1016/j.bbrc.2014.01.186
Journal or Publication Title:	Biochem. Biophys. Res. Commun.
Volume:	445
Number:	1
Page Range:	S. 244 - 250
Date:	2014
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	1090-2104
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CHROMAFFIN GRANULES; ANXA7 EXPRESSION; MURINE MODEL; CALCIUM; VII; CALPACTIN; CELLS Multiple languages Biochemistry & Molecular Biology; Biophysics Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44563