Universität zu Köln

Spahn, Philipp, Huelsmann, Sven, Rehorn, Klaus-Peter, Mischke, Stefanie, Mayer, Melanie, Casali, Andreu ORCID: 0000-0003-1738-869X and Reuter, Rolf (2014). Multiple regulatory safeguards confine the expression of the GATA factor Serpent to the hemocyte primordium within the Drosophila mesoderm. Dev. Biol., 386 (1). S. 272 - 280. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 1095-564X

Full text not available from this repository.

Abstract

serpent (sip) encodes a GATA-factor that controls various aspects of embryogenesis in Drosophila, such as fatbody development, gut differentiation and hematopoiesis. During hematopoiesis, sip expression is required in the embryonic head mesoderm and the larval lymph gland, the two known hematopoietic tissues of Drosophila, to obtain mature hemocytes. sip expression in the hemocyte primordium is known to depend on snail and buttonhead, but the regulatory complexity that defines the primordium has not been addressed yet. Here, we find that sip is sufficient to transform trunk mesoderm into hemocytes. We identify two disjoint cis-regulatory modules that direct the early expression in the hemocyte primordium and the late expression in mature hemocytes and lymph gland, respectively. During embryonic hematopoiesis, a combination of snail, buttonhead, empty spiracles and even-skipped confines the mesodermal sip expression to the head region. This restriction to the head mesoderm is crucial as ectopic sip in mesodermal precursors interferes with the development of mesodermal derivates and promotes hemocytes and fatbody development. Thus, several genes work in a combined fashion to restrain early srp expression to the head mesoderm in order to prevent expansion of the hemocyte primordium. (C) 2013 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Spahn, Philipp UNSPECIFIED UNSPECIFIED UNSPECIFIED Huelsmann, Sven UNSPECIFIED UNSPECIFIED UNSPECIFIED Rehorn, Klaus-Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Mischke, Stefanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Mayer, Melanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Casali, Andreu UNSPECIFIED orcid.org/0000-0003-1738-869X UNSPECIFIED Reuter, Rolf UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-447290
DOI:	10.1016/j.ydbio.2013.12.012
Journal or Publication Title:	Dev. Biol.
Volume:	386
Number:	1
Page Range:	S. 272 - 280
Date:	2014
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	1095-564X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FAT-BODY; DORSOVENTRAL PATTERN; EMPTY SPIRACLES; GENE-EXPRESSION; CELL-DEATH; HEMATOPOIESIS; HEAD; MELANOGASTER; ELEMENTS; IDENTIFICATION Multiple languages Developmental Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44729