Universität zu Köln

Sansone, Alfredo ORCID: 0000-0002-1197-7322, Hassenkloever, Thomas, Syed, Adnan S., Korsching, Sigrun I. ORCID: 0000-0002-5450-172X and Manzini, Ivan ORCID: 0000-0002-3575-9637 (2014). Phospholipase C and Diacylglycerol Mediate Olfactory Responses to Amino Acids in the Main Olfactory Epithelium of an Amphibian. PLoS One, 9 (1). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

The semi-aquatic lifestyle of amphibians represents a unique opportunity to study the molecular driving forces involved in the transition of aquatic to terrestrial olfaction in vertebrates. Most amphibians have anatomically segregated main and vomeronasal olfactory systems, but at the cellular and molecular level the segregation differs from that found in mammals. We have recently shown that amino acid responses in the main olfactory epithelium (MOE) of larval Xenopus laevis segregate into a lateral and a medial processing stream, and that the former is part of a vomeronasal type 2 receptor expression zone in the MOE. We hypothesized that the lateral amino acid responses might be mediated via a vomeronasal-like transduction machinery. Here we report that amino acid-responsive receptor neurons in the lateral MOE employ a phospholipase C (PLC) and diacylglycerol-mediated transduction cascade that is independent of Ca2+ store depletion. Furthermore, we found that putative transient receptor potential (TRP) channel blockers inhibit most amino acid-evoked responses in the lateral MOE, suggesting that ion channels belonging to the TRP family may be involved in the signaling pathway. Our data show, for the first time, a widespread PLC-and diacylglycerol-dependent transduction cascade in the MOE of a vertebrate already possessing a vomeronasal organ.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sansone, Alfredo UNSPECIFIED orcid.org/0000-0002-1197-7322 UNSPECIFIED Hassenkloever, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Syed, Adnan S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Korsching, Sigrun I. UNSPECIFIED orcid.org/0000-0002-5450-172X UNSPECIFIED Manzini, Ivan UNSPECIFIED orcid.org/0000-0002-3575-9637 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-448214
DOI:	10.1371/journal.pone.0087721
Journal or Publication Title:	PLoS One
Volume:	9
Number:	1
Date:	2014
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	Faculty of Mathematics and Natural Sciences > Department of Biology > Institute for Genetics
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language XENOPUS-LAEVIS TADPOLES; RECEPTOR POTENTIAL CHANNEL; SIGNAL-TRANSDUCTION; ODORANT RECEPTOR; VOMERONASAL NEURONS; SENSORY NEURONS; SYSTEM; TRPC2; ORGAN; ORGANIZATION Multiple languages Multidisciplinary Sciences Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/44821