Universität zu Köln

Patel, Trushar R. ORCID: 0000-0003-0627-2923, Bernards, Claudia, Meier, Markus ORCID: 0000-0003-1068-746X, McEleney, Kevin, Winzor, Donald J., Koch, Manuel ORCID: 0000-0002-2962-7814 and Stetefeld, Joerg ORCID: 0000-0003-1478-3248 (2014). Structural elucidation of full-length nidogen and the laminin-nidogen complex in solution. Matrix Biol., 33. S. 60 - 68. AMSTERDAM: ELSEVIER. ISSN 1569-1802

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Abstract

Nidogen-1 is a key basement membrane protein that is required for many biological activities. It is one of the central elements in organizing basal laminae including those in the skin, muscle, and the nervous system. The self-assembling extracellular matrix that also incorporates fibulins, fibronectin and integrins is clamped together by networks formed between nidogen, perlecan, laminin and collagen IV. To date, the full-length version of nidogen-1 has not been studied in detail in terms of its solution conformation and shape because of its susceptibility to proteolysis. In the current study, we have expressed and purified full-length nidogen-1 and have investigated its solution behavior using size-exclusion chromatography (SEC), dynamic light scattering (DLS) and small angle X-ray scattering (SAXS). The ab initio shape reconstruction of the complex between nidogen-1 and the laminin gamma-1 short arm confirms that the interaction is mediated solely by the C-terminal domains: the rest of the domains of both proteins do not participate in complex formation. (C) 2013 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Patel, Trushar R. UNSPECIFIED orcid.org/0000-0003-0627-2923 UNSPECIFIED Bernards, Claudia UNSPECIFIED UNSPECIFIED UNSPECIFIED Meier, Markus UNSPECIFIED orcid.org/0000-0003-1068-746X UNSPECIFIED McEleney, Kevin UNSPECIFIED UNSPECIFIED UNSPECIFIED Winzor, Donald J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Koch, Manuel UNSPECIFIED orcid.org/0000-0002-2962-7814 UNSPECIFIED Stetefeld, Joerg UNSPECIFIED orcid.org/0000-0003-1478-3248 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-450566
DOI:	10.1016/j.matbio.2013.07.009
Journal or Publication Title:	Matrix Biol.
Volume:	33
Page Range:	S. 60 - 68
Date:	2014
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	1569-1802
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language X-RAY-SCATTERING; MEMBRANE GLYCOPROTEIN ENTACTIN; EGF-LIKE MOTIF; GAMMA 1 CHAIN; CELL ATTACHMENT; BINDING; PROTEIN; GENE; MACROMOLECULES; LOCALIZATION Multiple languages Biochemistry & Molecular Biology; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/45056