Universität zu Köln

Hoerster, Robert, Muether, Philipp S., Vierkotten, Sarah, Hermann, Manuel M., Kirchhof, Bernd and Fauser, Sascha (2014). Upregulation of TGF-1 in experimental proliferative vitreoretinopathy is accompanied by epithelial to mesenchymal transition. Graefes Arch. Clin. Exp. Ophthalmol., 252 (1). S. 11 - 17. NEW YORK: SPRINGER. ISSN 1435-702X

Full text not available from this repository.

Abstract

Proliferative vitreoretinopathy (PVR) is characterized by epithelial to mesenchymal transition (EMT) of retinal pigment epithelium (RPE) cells and consecutive formation of fibrous membranes, leading to retinal redetachment. Transforming growth factor beta (TGF-) has been suggested to play an important role in this process, but the role of TGF- isoforms is unknown. In pigmented rabbits (n = 14), PVR was induced by cryopexy and a full-thickness limbus-parallel incision. PVR was evaluated by indirect ophthalmoscopy. Concentrations of TGF- isoforms were determined by multiplex bead assay analysis in aqueous humor (AH) and vitreous samples. EMT marker vimentin was analyzed by western blot. Masson's-trichrome, haematoxilin and eosine (H&E), and immunohistochemical analysis for EMT marker alpha SMA were performed on cross-sections of eyes. PVR was induced in all treated eyes. The number of quadrants affected by PVR was 1 (n = 5), 2 (n = 2), 3 (n = 2), 4 (n = 5). Vimentin and alpha SMA were expressed during PVR development. During PVR development, both TGF-1 levels (AH: p = 0.001; vitreous: p = 0.002) and TGF-2 levels increased (AH: p = 0.027; vitreous: p = 0.02), while TGF-3 was not detected at any timepoint. The increase was more pronounced for TGF-1 than for TGF- 2 (AH: p = 0.002; vitreous: p = 0.0005), and only TGF-1 correlated with the amount of PVR (p = 0.024, r = 0,723). Development of PVR membranes was accompanied by a pronounced upregulation of TGF-1, rather than TGF-2. Therefore TGF-1 could be a promising target for inhibition of PVR.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hoerster, Robert UNSPECIFIED UNSPECIFIED UNSPECIFIED Muether, Philipp S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Vierkotten, Sarah UNSPECIFIED UNSPECIFIED UNSPECIFIED Hermann, Manuel M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kirchhof, Bernd UNSPECIFIED UNSPECIFIED UNSPECIFIED Fauser, Sascha UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-451344
DOI:	10.1007/s00417-013-2377-5
Journal or Publication Title:	Graefes Arch. Clin. Exp. Ophthalmol.
Volume:	252
Number:	1
Page Range:	S. 11 - 17
Date:	2014
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1435-702X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RETINAL-DETACHMENT; CELLS; TRACTION; BETA Multiple languages Ophthalmology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/45134