Universität zu Köln

Lintala, Minna, Schuck, Natalie, Thormaehlen, Ina, Jungfer, Andreas, Weber, Katrin L., Weber, Andreas P. M., Geigenberger, Peter ORCID: 0000-0001-9512-349X, Soll, Juergen, Boelter, Bettina and Mulo, Paula ORCID: 0000-0002-8728-3204 (2014). Arabidopsis tic62 trol Mutant Lacking Thylakoid-Bound FerredoxinNADP Oxidoreductase Shows Distinct Metabolic Phenotype. Mol. Plant., 7 (1). S. 45 - 58. OXFORD: OXFORD UNIV PRESS. ISSN 1752-9867

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Abstract

Ferredoxin-NADP(+) oxidoreductase (FNR), functioning in the last step of the photosynthetic electron transfer chain, exists both as a soluble protein in the chloroplast stroma and tightly attached to chloroplast membranes. Surface plasmon resonance assays showed that the two FNR isoforms, LFNR1 and LFNR2, are bound to the thylakoid membrane via the C-terminal domains of Tic62 and TROL proteins in a pH-dependent manner. The tic62 trol double mutants contained a reduced level of FNR, exclusively found in the soluble stroma. Although the mutant plants showed no visual phenotype or defects in the function of photosystems under any conditions studied, a low ratio of NADPH/NADP(+) was detected. Since the CO2 fixation capacity did not differ between the tic62 trol plants and wild-type, it seems that the plants are able to funnel reducing power to most crucial reactions to ensure survival and fitness of the plants. However, the activity of malate dehydrogenase was down-regulated in the mutant plants. Apparently, the plastid metabolism is able to cope with substantial changes in directing the electrons from the light reactions to stromal metabolism and thus only few differences are visible in steady-state metabolite pool sizes of the tic62 trol plants.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lintala, Minna UNSPECIFIED UNSPECIFIED UNSPECIFIED Schuck, Natalie UNSPECIFIED UNSPECIFIED UNSPECIFIED Thormaehlen, Ina UNSPECIFIED UNSPECIFIED UNSPECIFIED Jungfer, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Weber, Katrin L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Weber, Andreas P. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Geigenberger, Peter UNSPECIFIED orcid.org/0000-0001-9512-349X UNSPECIFIED Soll, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Boelter, Bettina UNSPECIFIED UNSPECIFIED UNSPECIFIED Mulo, Paula UNSPECIFIED orcid.org/0000-0002-8728-3204 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-452525
DOI:	10.1093/mp/sst129
Journal or Publication Title:	Mol. Plant.
Volume:	7
Number:	1
Page Range:	S. 45 - 58
Date:	2014
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1752-9867
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ADP-GLUCOSE PYROPHOSPHORYLASE; PEA LEAF CHLOROPLASTS; POSTTRANSLATIONAL REDOX-MODIFICATION; NADP-MALATE DEHYDROGENASE; STARCH SYNTHESIS; ENZYME COLOCALIZATION; LIGHT ACTIVATION; BINDING-PROTEIN; REDUCTASE; COMPLEX Multiple languages Biochemistry & Molecular Biology; Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/45252