Universität zu Köln

Wagner, Stephan ORCID: 0000-0001-5369-7911, Stuttmann, Johannes ORCID: 0000-0002-6207-094X, Rietz, Steffen, Guerois, Raphael, Brunstein, Elena, Bautor, Jaqueline, Niefind, Karsten ORCID: 0000-0002-0183-6315 and Parker, Jane E. (2013). Structural Basis for Signaling by Exclusive EDS1 Heteromeric Complexes with SAG101 or PAD4 in Plant Innate Immunity. Cell Host Microbe, 14 (6). S. 619 - 631. CAMBRIDGE: CELL PRESS. ISSN 1934-6069

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Abstract

Biotrophic plant pathogens encounter a postinfection basal resistance layer controlled by the lipase-like protein enhanced disease susceptibility 1 (EDS1) and its sequence-related interaction partners, senescence-associated gene 101 (SAG101) and phytoalexin deficient 4 (PAD4). Maintainance of separate EDS1 family member clades through angiosperm evolution suggests distinct functional attributes. We report the Arabidopsis EDS1-SAG101 heterodimer crystal structure with juxtaposed N-terminal alpha/beta hydrolase and C-terminal alpha-helical EP domains aligned via a large conserved interface. Mutational analysis of the EDS1-SAG101 heterodimer and a derived EDS1-PAD4 structural model shows that EDS1 signals within mutually exclusive heterocomplexes. Although there is evolutionary conservation of alpha/beta hydrolase topology in all three proteins, a noncatalytic resistance mechanism is indicated. Instead, the respective N-terminal domains appear to facilitate binding of the essential EP domains to create novel interaction surfaces on the heterodimer. Transitions between distinct functional EDS1 heterodimers might explain the central importance and versatility of this regulatory node in plant immunity.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wagner, Stephan UNSPECIFIED orcid.org/0000-0001-5369-7911 UNSPECIFIED Stuttmann, Johannes UNSPECIFIED orcid.org/0000-0002-6207-094X UNSPECIFIED Rietz, Steffen UNSPECIFIED UNSPECIFIED UNSPECIFIED Guerois, Raphael UNSPECIFIED UNSPECIFIED UNSPECIFIED Brunstein, Elena UNSPECIFIED UNSPECIFIED UNSPECIFIED Bautor, Jaqueline UNSPECIFIED UNSPECIFIED UNSPECIFIED Niefind, Karsten UNSPECIFIED orcid.org/0000-0002-0183-6315 UNSPECIFIED Parker, Jane E. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-470180
DOI:	10.1016/j.chom.2013.11.006
Journal or Publication Title:	Cell Host Microbe
Volume:	14
Number:	6
Page Range:	S. 619 - 631
Date:	2013
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	1934-6069
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ALPHA/BETA-HYDROLASE FOLD; DISEASE-RESISTANCE GENES; NB-LRR GENES; ARABIDOPSIS EDS1; RECEPTOR GID1; CELL-DEATH; PROTEINS; STRIGOLACTONE; RECOGNITION; PERCEPTION Multiple languages Microbiology; Parasitology; Virology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47018